产品说明
应用
Invertase Glycoprotein Standard has been used:
- to generate N-linked glycan library
- as a negative control to study the binding of lectins to high mannose structures
- for sample pre-treatment in proteomic analyses to study drug-induced toxic epidermal necrolysis
The Invertase Glycoprotein Standard can be used to demonstrate N-glycosylation using PNGase F with both in-solution and in-gel procedures. The extent of deglycosylation can be assessed by mobility shift on SDS-PAGE gels.
用于生产糖果食品和人造蜂蜜。
生化/生理作用
转化酶将蔗糖水解成葡萄糖和果糖,产生无色产物,与产生有色产物的酸水解不同。
其他说明
Invertase is an enzyme that catalyses the hydrolysis of sucrose into fructose and glucose. Invertase Glycoprotein Standard is the periplasmic (glycosylated form, external invertase) with 50% of its mass as polymannan. Since yeast can provide an alternative system for protein glycosylation that is similar to mammalian systems, periplasmic invertase is often used as a model for the study of the function of oligosaccharides in glycoproteins and for studies on glycoprotein biosynthesis.
基本信息
酶学委员会(EC)编号 | 3.2.1.26 ( BRENDA | IUBMB ) |
EC 号 | 232-615-7 |
MDL编号 | MFCD00131410 |
NACRES | NA.32 |
产品性质
质量水平 | 200 |
生物来源 | Saccharomyces cerevisiae |
等级 | for proteomics |
产品线 | BioReagent |
测定 | ≥0.5 mg/vial protein basis (E |
形式 | lyophilized powder |
分子量 | 60 kDa |
储存温度 | 2-8℃ |
安全信息
象形图 | |
警示用语: | Danger |
危险声明 | H334 |
预防措施声明 | P261 - P284 - P501 |
危险分类 | Resp. Sens. 1 |
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 3 |
个人防护装备 | Eyeshields, Gloves, type N95 (US) |