Anti-PNMT Antibody

产品说明

一般描述

PNMT, also known as Phosphatidylethanolamine N-methyltransferase, or PEMT, PEAMT, or PEMT2, and encoded by the gene PEMT/PEMPT/PNMT, catalyzes three sequential methylation reactions of phosphatidylethanolamine to produce phosphatidylcholine a major constituent of cell membranes and a primary pulmonary surfactant. PNMT/PEMT is localized to the endoplasmic reticulum membrane as well as the mitochondrial membranes. PNMT/PEMT is widely expressed, and has the highest activity in the mitochondria. The conversion of phosphatidylethanolamine to phosphatidylcholine by sequential methylation via PNMT/PEMT occurs in the liver. A different pathway for phosphatidylcholine production starting from choline also is present in cells but it does not involve PNMT/PEMT enzyme. Mutations in PNMT/PEMT protein are associated with nonalcoholic fatty liver disease (NAFLD).

免疫原

KLH-conjugated linear peptide corresponding to the C-terminus of human PNMT.
Epitope: C-terminus

应用

Immunohistochemistry Analysis: A 1:500 dilution and a 1:2,000 dilution from a representative lot detected PNMT in human adrenal gland and testis tissues, respectively.
Research Sub Category
Signaling Neuroscience
Research Category
Neuroscience
Anti-PNMT Antibody detects level of PNMT & has been published & validated for use in PNMT.

质量

Evaluated by Western Blotting in human liver tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected PNMT in 10 µg of human liver tissue lysate.

目标描述

~17 kDa observed. As isoform of PNMT has been observed between 15-20 kDa (Cui, Z., et al. (1993). J Biol. Chem. 268(22):16655-16663.).

联系

Replaces: AB110

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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