产品说明
一般描述
Histone deacetylases (HDACs) are competing enzymes, belonging to histone deacetylase family. There are two classes of HDACs with six to seven different types of HDACs proteins. HDAC1,HDAC2, HDAC3, and HDAC8 belong to Class I HDACs and HDAC4, HDAC6, HDAC7, HDAC9, and HDAC10 belong to Class II HDACs. Class I HDACs consists of a single deacetylase domain at the N-termini and diversified C-terminal regions, while Class II contains a deacetylase domain at C-terminal position. Studies show that HDAC8 is a sex-linked gene located on the chromosome at position Xq21.2 - q21.3. HDAC8 gene has a molecular weight of 43kDa and it encodes a 377 amino acid protein. It is present within the nucleas. HDAC8 mRNA is seen in heart, lung, kidney, and pancreas.
The antibody is affinity-purified using the immunizing peptide immobilized on agarose.
免疫原
synthetic peptide corresponding to amino acid residues 352-370 of human HDAC8, conjugated to KLH. The corresponding sequence in mouse differs by one amino acid.
应用
Anti-Histone Deacetylase 8 (HDAC8) antibody produced in rabbit has been used in western blotting, immunohistofluorescence and immunoblotting.
生化/生理作用
Histone deacetylation results in transcription repression leading to the formation of tight nucleosomal structure which prevents DNA accessing. {5} HDAC8 controls HDAC activity on H4 histone peptide substrates. HDAC8 is similar to the HDAC class I enzymes. In vitro expression and activity of HDAC8 was examined using FLAG tagged- HDAC8 and HDAC1. These were transfected into HeLa cells and Sf9 insect cells. After the immunoprecipitation of cell lysates the expression results were confirmend using Western blotting. Studies show that HDAC8 may play a role in role in transcriptional regulation and could possibly be regulated in a temporal or compartment-specific manner.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD04975119 |
产品性质
| 生物来源 | rabbit |
| 质量水平 | 200 |
| 偶联物 | unconjugated |
| 抗体形式 | affinity isolated antibody |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~43 kDa |
| species reactivity | human, mouse |
| 增强验证 | recombinant expression Learn more about Antibody Enhanced Validation |
| technique(s) | immunocytochemistry: 1-2 μg/mL using cultured 293T cells expressing recombinant human HDAC8 immunoprecipitation (IP): 1-2 μg using extract of 293T cells expressing recombinant human HDAC8 western blot: 2-4 μg/mL using whole extract of human colon adenocarcinoma SW620 cells and a chemiluminescent detection reagent |
| UniProt登记号 | Q9BY41 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... HDAC8(55869) mouse ... Hdac8(70315) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | nwg |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
