产品说明
一般描述
Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site and a generic tyrosine phosphorylated peptide, followed by immunoaffinity chromatography. Recognizes the ~185 KDa EGFR protein phosphorylated on Tyr845.
Recognizes the ~170 kDa EGFR protein phosphorylated at Tyr845 in EGF-treated A431 cells. Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
This PhosphoDetect
免疫原
a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr⁸⁴⁵ phosphorylation site of human EGFR
包装
10 tests in Plastic ampoule
应用
Immunoblotting (1:1000)
警告
Toxicity: Standard Handling (A)
外形
In Dulbecco′s PBS (w/o Mg2+ and Ca2+), 1 mg/ml BSA, 50% glycerol, pH 7.4.
重悬
Following initial thaw, aliquot and freeze (-70°C).
分析说明
Positive Control
A431 cells stimulated with EGF
其他说明
Sieg, D.J. et al. 2000. Nat. Cell. Biol.2, 249.
Barbier, A.J. et al. 1999. J. Biol. Chem.274, 14067.
Biscardi, J.S., et al. 1999. J. Biol. Chem.274, 8335.
Hashimoto, A. et al. 1999. J. Biol. Chem.274, 20139
Poppleton, H.M. et al. 1999. Arch. Biochem. Biophys.363, 227.
The immunogen sequence is 100% conserved in mouse and rat and 93% conserved in chicken EGFR, but cross-reactivity has not been tested. When diluted to 1:1000 there is sufficient antibody to perform 10 miniblots (10 T) at 10 ml per blot. Variables associated with assay conditions will dictate the proper working dilution.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
| 生物来源 | rabbit |
| 质量水平 | 100 |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 形式 | liquid |
| 包含 | ≤0.1% sodium azide as preservative |
| species reactivity | human |
| manufacturer/tradename | Calbiochem® |
| 储存条件 | OK to freeze avoid repeated freeze/thaw cycles |
| 同位素/亚型 | IgG |
| 运输 | wet ice |
| 储存温度 | −70℃ |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
