产品说明
应用
5-Chloro-2′-deoxyuridine has been used to:
- study cell cycle dynamics by immunohistochemical analysis and double S-phase labeling using animal models
- analyze cell cycle length using an animal model
- label HEK293T cells for molecular combing assay
包装
100 mg in poly bottle
生化/生理作用
DNA labeled with 5-chloro-2′-deoxyuridine (CldU) serves as an effective tool to analyze and quantify DNA replication, repair, and recombination. CldU is a potent mutagen, clastogen, and toxicant. It is used as a thymidine analog and is found to alter the dNTP pools and might lead to cell-cycle arrest. CldU produces sister-chromatid exchange but has less response to ionizing radiation compared to other thymine analogs. 5-Chloro-2′-deoxyuridine (CldU) is used to study the miscoding potential of hypochlorous acid damage to DNA and DNA precursors. When used with antibody based immunofluorescent imaging, 5-Chloro-2′-deoxyuridine incorporation may be used in protocols to identify sites of DNA replication. CldU may be used as a labeling substrate in conjunction with other halogenated uridine labeling substrates such as iododeoxyuridine (IdU).
基本信息
| 经验(实验)分子式 | C9H11ClN2O5 |
| 分子量 | 262.65 |
| MDL编号 | MFCD00006531 |
| PubChem化学物质编号 | 24892910 |
| NACRES | NA.51 |
产品性质
| 质量水平 | 100 |
| 测定 | ≥98% (HPLC) |
| 形式 | powder |
| 溶解性 | 1 M NH4OH: 20 mg/mL, clear, colorless to faintly yellow |
| 储存温度 | −20℃ |
| SMILES string | OC[C@H]1O[C@H](C[C@@H]1O)N2C=C(Cl)C(=O)NC2=O |
| InChI | 1S/C9H11ClN2O5/c10-4-2-12(9(16)11-8(4)15)7-1-5(14)6(3-13)17-7/h2,5-7,13-14H,1,3H2,(H,11,15,16)/t5-,6+,7+/m0/s1 |
| InChI key | NJCXGFKPQSFZIB-RRKCRQDMSA-N |
安全信息
| 象形图 |   |
| 警示用语： | Warning |
| 危险声明 | H302 + H312 + H332 - H351 |
| 预防措施声明 | P202 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312 - P308 + P313 |
| 危险分类 | Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 2 |
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | dust mask type N95 (US), Eyeshields, Gloves |
