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Anti-Growth Factor Independence-1 antibody, Mouse monoclonal

品牌
Sigma-Aldrich
货号
G6670
规格纯度
clone 2.5D.17, purified from hybridoma cell culture
参考价格
6923.34 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Monoclonal Anti-Growth Factor Independence-1 (GFI1) (mouse IgG1 isotype) is derived from the hybridoma 2.5D.17 produced by the fusion of mouse myeloma cells (SP2 cells) and splenocytes from BALB/c mice immunized with rat GFI1. Growth Factor Independence 1 (GFI1) is a zinc-finger transcriptional repressor, that is located on human chromosome 1p22. Gfi1 is highly expressed in thymus while Gfi1B is the predominant factor expressed in spleen, and both are expressed in bone marrow. It has a repression domain named SNAG at the N-terminal.† The protein also has a zinc finger DNA binding domain that is homologous to the domain in Gfi1B. Both bind to same consensus DNA sequence

特异性

The antibody epitope resides within the GFI1 SNAG transcriptional-repression domain (amino acids 7-26).

免疫原

rat growth factor independence-1 (GFI1).

应用

Monoclonal Anti-Growth Factor Independence-1 (GFI1) antibody may be used in:

  • immunoblotting
  • immunohistochemistry
  • EMSA (electrophoretic mobility shift analysis)

生化/生理作用

Growth factor independence-1 (Gfi1) plays a key role in hematopoiesis along with Gfi1B proteins. Cytokines that regulate innate immunity induce Gfi1 expression. Deficiency of this protein causes thymic and peripherial T lymphopenia and severe abnormalities in pre-T-cell development in mice. Mutations in this gene cause defects in T lymphocyte function and development in humans. Gfi1 autoregulates its expression in T lymphocytes.

外形

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

分析说明

The antibody may also be used in EMSA (electrophoretic mobility shift analysis).

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号MFCD02263329
NACRESNA.41

产品性质

质量水平200
生物来源mouse
偶联物unconjugated
抗体形式purified from hybridoma cell culture
antibody product typeprimary antibodies
克隆2.5D.17, monoclonal
形式buffered aqueous solution
分子量antigen ~55 kDa
species reactivitymouse, rat, human
浓度~2 mg/mL
technique(s)immunohistochemistry: suitable
microarray: suitable
western blot: 1-2 μg/mL using total cell extract of A549 cells
同位素/亚型IgG1
UniProt登记号Q99684
运输dry ice
储存温度−20℃
Gene Informationhuman ... GFI1(2672)
mouse ... Gfi1(14581)
rat ... Gfi1(24388)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable
个人防护装备Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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