产品说明
一般描述
Glycerol 3-phosphate Oxidase is a FAD-dependent α-glycerophosphate oxidase and catalyzes the formation of dihydroxyacetonephosphate. Glycerol 3-phosphate oxidase comprises of a flavin adenine dinucleotide (FAD) and a substrate binding domain and corresponds to molecular weight of 42 kDa. It exists as a monomer and the active site comprises of isoalloxazine ring and histidine 53, arginine 316 and lysine 345 residues crucial for ligand binding.
应用
Glycerol 3-phosphate Oxidase from Pediococcus sp. has been used in the kinetic assay to study gluconeogenesis in liver and in immobilization studies on pencil graphite (PG) electrode.
This enzyme is useful for enzymatic determination of triglyceride when coupled with lipoprotein lipase (LPL-311, LPL-314) and glycerokinase (GYK-301, GYK-311) in clinical analysis.
包装
100 units in glass bottle
物理属性
Isoelectric point : 4.1 -/+0.1
Michaelis constants : 3.2x10 -3M (L-α-Glycerophosphate), 6.8 x 10 -3M (D, L-form)
Inhibitors : Ionic detergents (SDS, LBS, etc.), Hg++, Ag+
Optimum pH : 8.0 - 8.5Optimum temperature : 35 - 40oC
pH Stability : pH 6.5 - 8.5 (25oC, 20hr) Thermal stability : below 40oC (pH 7.0, 15min)
Substrate specificity : The enzyme has the highest specificity for L-form of a-γlycerophosphate
单位定义
One unit will oxidize 1.0 μmole of
外形
Lyophilized powder containing stabilizers
基本信息
酶学委员会(EC)编号 | 1.1.3.21 ( BRENDA | IUBMB ) |
EC 号 | 232-932-0 |
MDL编号 | MFCD00131209 |
NACRES | NA.54 |
产品性质
形式 | lyophilized powder |
质量水平 | 200 |
specific activity | 40-80 units/mg solid, pH 8.1 |
分子量 | ~76 kDa (gel filtration) |
储存温度 | 2-8℃ |
安全信息
储存分类代码 | 13 - Non Combustible Solids |
WGK | WGK 3 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
个人防护装备 | Eyeshields, Gloves, type N95 (US) |