产品说明
一般描述
Fpg contains two domains separated by a flexible hinge.
Formamidopyrimidine-DNA glycosylase (Fpg) is a DNA repair enzyme found in Escherichia coli, which contains one zinc atom. Proximal to its C-terminal, it contains a zinc-finger motif of CC/CC type.
应用
Fpg Protein from Escherichia coli has been used for the assessment of DNA oxidative damage using comet assay.
包装
10 μg in poly bottle
生化/生理作用
Fpg is a key enzyme in the DNA base excision repair pathway (BER). It catalyses the excision of a broad spectrum of modified purines. Fpg has both DNA glycosylase activity that removes the mutated base and AP-lyase activity that releases ribose leaving both 5′- and 3′-phosphorylated ends in the DNA. The zinc finger motif at its C-terminus is responsible for the DNA binding and AP-lyase activity. In addition, its N-terminal proline acts as a nucleophile to produce a Schiff base intermediate that is essential for enzyme action.
Fpg specifically acts on 3′- and 5′-phosphodiester bonds.
Formamidopyrimidine-DNA glycosylase (Fpg) cleaves double-stranded DNA containing the damaged base 8-oxo-7,8-dihydroguanine. It functions as an N-glycosylase and apurinic/apyrimidinic lyase.
单位定义
One unit will cleave 50% of 0.5 pmol of double-stranded DNA oligomer substrate (8-oxoguanine−mutated) in 10 min at 25 ℃.
外形
Solution in 50% glycerol containing 50 mM potassium HEPES, pH 7.5, 1 mM DTT, 1 mM EDTA, and 200 mM NaCl.
基本信息
| 酶学委员会(EC)编号 | 3.2.2.23 ( BRENDA | IUBMB ) |
| MDL编号 | MFCD02264758 |
| NACRES | NA.32 |
产品性质
| 质量水平 | 200 |
| 生物来源 | Escherichia coli |
| 重组 | expressed in E. coli |
| 测定 | ≥90% (SDS-PAGE) |
| 形式 | buffered aqueous glycerol solution |
| specific activity | >20,000 units/mg protein |
| 分子量 | 30.2 kDa (269 amino acids, predicted from the nucleotide sequence) |
| UniProt登记号 | P05523 |
| application(s) | genomic analysis |
| 运输 | wet ice |
| 储存温度 | −20℃ |
| Gene Information | Escherichia coli CFT073 ... mutM(1038243) Escherichia coli K12 ... mutM(946765) |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 2 |
| 个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
