产品说明
应用
Deoxyribonuclease II from porcine spleen has been used in an immunohistological study of the immune system cells in paraffin-embedded tissues. Deoxyribonuclease II from porcine spleen has also been used in a study to investigate its reassociation with the lysosomal membrane.
DNase II from Sigma has been used to treat transformed cells during the purification of β-lactamase. It has also been used for the preparation of adenoma tissue in a study that investigated the effect of somatoprim on growth hormone secretion in human adenoma cell cultures (hSA).
包装
20000, 80000 units in poly bottle
生化/生理作用
The enzyme is a lysosomal acid hydrolase that hydrolyzes DNA to 3′-phosphoryl oligonucleotides in the absence of metal ions, under acidic conditions. It is a non-covalently linked α·β heterodimer of approximately 45,000 Da molecular mass.
单位定义
One Kunitz unit will produce a ΔA260 of 0.001 per min per mL at pH 4.6 at 25℃; [Mg2+] = 0.83 mM
外形
含氯化钠
基本信息
| 酶学委员会(EC)编号 | 3.1.22.1 ( BRENDA | IUBMB ) |
| EC 号 | 232-801-8 |
| MDL编号 | MFCD00130919 |
| NACRES | NA.54 |
产品性质
| 质量水平 | 200 |
| 类型 | Type IV |
| 形式 | lyophilized powder |
| specific activity | 2,000-6,000 Kunitz units/mg protein (biuret) |
| 分子量 | 45 kDa |
| 组成 | Protein, 60-80% |
| 异质活性 | RNase ≤0.05% |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
