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Monoclonal Anti-Dihydropyridine Receptor (α2 Subunit) antibody produced in mouse

品牌
Sigma-Aldrich
货号
D219
规格纯度
clone 20A, ascites fluid, buffered aqueous solution
参考价格
11010.8 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Dihydropyridine Receptor (DHPR) is a heteropentamer calcium ion channel and comprises of α1,α2/δ,β and γ subunits. It is localized in the skeletal muscle in the t-tubule membrane. The α2subunit of DHPR is a glycosylated subunit and is anchored on the membrane.

特异性

Reacts specifically with the α2 subunit of the DHP receptor. Strong reactivity is observed with the α2 subunit in rat, mouse, guinea pig, rabbit skeletal and cardiac muscle, and rat brain and lung tissue. Weaker reactivity is observed with rat kidney, liver and spleen tissues. By immunohistochemistry, double rows of discrete punctate staining represent pairs of triads on the opposing sides of the Z-lines.

免疫原

Rabbit dihydropyridine (DHP) receptors, (α2 subunit)

应用

Use of this antibody for immunoblot analysis will allow for detection of a 220 kDa band (native conditions) or a 143 kDa band (denaturing conditions).Use of this antibody for immunohistochemistry of muscle tissue will demonstrate double rows of discrete punctate staining representing pairs of triads on the opposing sides of the Z-lines are observed.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Monoclonal Anti-Dihydropyridine Receptor (α2 Subunit) antibody produced in mouse has been used in:

  • immunofluorescent histochemistry
  • surface plasmon resonance binding
  • immunohistochemistry (IHC)
  • immunoblotting

生化/生理作用

Dihydropyridine Receptor (DHFR) α1 subunit is the voltage-sensor. DHPR interacts with ryanodine receptor (RyR) and also mediates excitation-contraction (EC) coupling in cardiac and skeletal muscles. Mutation in the DHPR (E1014K) affects its affinity and selectivity towards calcium permeation. Additionally, it also plays a key role in the synaptic transmission.

外形

Solution in phosphate buffered saline containing 0.05% sodium azide.

其他说明

This mouse monoclonal IgG2a antibody is supplied as a mouse ascites diluted in phosphate buffered saline containing 0.05% sodium azide as a preservative.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号MFCD01323189
NACRESNA.41

产品性质

质量水平200
生物来源mouse
偶联物unconjugated
抗体形式ascites fluid
antibody product typeprimary antibodies
克隆20A, monoclonal
形式buffered aqueous solution
分子量antigen 143 kDa (reduced)
antigen 220 kDa (non-reduced)
species reactivityguinea pig, rabbit, mouse, rat
technique(s)immunohistochemistry (frozen sections): 1:500
western blot: 1:500
同位素/亚型IgG2a
UniProt登记号O08532
运输dry ice
储存温度−20℃
Gene Informationmouse ... Cacna2d1(12293)
rat ... Cacna2d1(25399)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKnwg
闪点(F)Not applicable
闪点(C)Not applicable
个人防护装备Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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