产品说明

一般描述

Middlebrook 7H9 Broth Base contains many inorganic salts, which support the growth of mycobacteria. Sodium citrate provides citrate ions in the medium, which retain inorganic cations in the solution.

应用

Middlebrook 7H9 Broth Base has been used for the cultivation Mycobacterium bovis bacillus Calmette-Guérin Pasteur strain. It is also recommended for cultivation and sensitivity testing of Mycobacterium tuberculosis.

组分

成分 (g/L)
硫酸铵，0.50
磷酸二钠，2.50
磷酸二氢钾，1.00
氯化钠，0.10
硫酸镁，0.05
氯化钙，0.0005
硫酸锌，0.001
硫酸铜，0.001
柠檬酸铁铵，0.04
L-谷氨酸，0.50
吡哆素，0.001
生物素，0.0005

制备说明

Suspend 2.35 g of Middlebrook 7H9 Broth Base in 450 ml of distilled water. Add either 2 ml of glycerol (Cat. No 49767) or 0.5 g of TWEEN^® 80 (Cat. No. 93780). Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. Pressure (121℃) for 10 minutes. Cool to 45℃ or below and aseptically add 1 vial of Middlebrook ADC Growth Supplement (Cat. No. M0553). Mix well before dispensing.

附注

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

法律信息

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC

基本信息

产品性质

安全信息