产品说明
一般描述
Amicon® Ultra-0.5 centrifugal filter devices provide fast ultrafiltration, with the capability for high concentration factors and easy concentrate recovery from dilute and complex sample matrices. For example, IgG (156 kDa) at 1 mg/ml initial concentration is typically retained at >95% .
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 30-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample (retained species) is achieved by a convenient reverse spin step after collecting the filtrate.
Amicon® Ultra-0.5 devices are supplied non-sterile and are for single use only. With their vertical membrane design and reverse spin, Amicon® Ultra 0.5 mL filters deliver great performance and lower spin times.
应用
- Ultrafiltration with 100,000 Dalton molecular weight cutoff Ultracel® membrane
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
- Purification of macromolecular components found in tissue culture extracts or cell lysates, primer removal, linkers, or macromolecular labels from a reaction mix, and protein removal prior to HPLC
- Desalting, buffer exchange, and protein dialysis
特点和优势
- Ultracel® regenerated cellulose membrane with 100 kDa NMWCO delivers retentate recovery of greater than 90%
- Concentrates 500 μL down to 15 μL
- Concentration factor 25x to 30x
- High sample recovery (greater than 90% of dilute starting solution)
- Fast processing time: 10 to 30 minutes
- Reverse spin capability provides consistent recoveries
联系
替代品：42414
外形
色码：透明
法律信息
Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
| eCl@ss | 36100101 |
| NACRES | NB.24 |
产品性质
| 物料 | polypropylene cap polypropylene tube regenerated cellulose membrane styrene-butadiene housing |
| 质量水平 | 400 |
| 无菌性 | non-sterile |
| 产品线 | Amicon® |
| manufacturer/tradename | Amicon® Ultra |
| technique(s) | protein extraction: suitable protein purification: suitable |
| 长度 | 4.99 cm (1.96 in.) |
| 直径 | 10.8 mm |
| 过滤面积 | 1 cm2 |
| 孔径 | 100 kDa NMWCO |
| 试验参数 | sample volume: 0.5 mL |
| 运输 | ambient |
安全信息
| 储存分类代码 | 10-13 - German Storage Class 10 to 13 |
