位置:首页 > 品牌 > Sigma-Aldrich >

Amicon Ultra-0.5 Centrifugal Filter Unit

Amicon Ultra-0.5 离心过滤装置

产品介绍:

产品说明

一般描述

Amicon® Ultra-0.5 centrifugal filter devices provide fast ultrafiltration, with the capability for high concentration factors and easy concentrate recovery from dilute and complex sample matrices. For example, IgG (156 kDa) at 1 mg/ml initial concentration is typically retained at >95% .

The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 30-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample (retained species) is achieved by a convenient reverse spin step after collecting the filtrate.

Amicon® Ultra-0.5 devices are supplied non-sterile and are for single use only. With their vertical membrane design and reverse spin, Amicon® Ultra 0.5 mL filters deliver great performance and lower spin times.

应用

  • Ultrafiltration with 100,000 Dalton molecular weight cutoff Ultracel® membrane
  • Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
  • Purification of macromolecular components found in tissue culture extracts or cell lysates, primer removal, linkers, or macromolecular labels from a reaction mix, and protein removal prior to HPLC
  • Desalting, buffer exchange, and protein dialysis

特点和优势

  • Ultracel® regenerated cellulose membrane with 100 kDa NMWCO delivers retentate recovery of greater than 90%
  • Concentrates 500 μL down to 15 μL
  • Concentration factor 25x to 30x
  • High sample recovery (greater than 90% of dilute starting solution)
  • Fast processing time: 10 to 30 minutes
  • Reverse spin capability provides consistent recoveries

联系

替代品:42414

外形

色码:透明

法律信息

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

基本信息

eCl@ss36100101
NACRESNB.24

产品性质

物料polypropylene cap
polypropylene tube
regenerated cellulose membrane
styrene-butadiene housing
质量水平400
无菌性non-sterile
产品线Amicon®
manufacturer/tradenameAmicon® Ultra
technique(s)protein extraction: suitable
protein purification: suitable
长度4.99 cm (1.96 in.)
直径10.8 mm
过滤面积1 cm2
孔径100 kDa NMWCO
试验参数sample volume: 0.5 mL
运输ambient

安全信息

储存分类代码10-13 - German Storage Class 10 to 13

Sigma-Aldrich

推荐产品
| 首页 | 联系我们 | 会员服务 | 广告服务 | 友情链接 |
版权所有 CopyRight © 2008-2024 粤ICP备08119708号