产品说明

一般描述

Amicon^® Ultra-0.5 centrifugal filter devices provide fast ultrafiltration, with the capability for high concentration factors and easy concentrate recovery from dilute and complex sample matrices. For example, Cytochrome c (12.4 kDa) at 0.25 mg/ml initial concentration is typically retained at >95% .

The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 30-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample (retained species) is achieved by a convenient reverse spin step after collecting the filtrate.

Amicon^® Ultra-0.5 devices are supplied non-sterile and are for single use only. With their vertical membrane design and reverse spin, Amicon^® Ultra 0.5 mL filters deliver great performance and lower spin times.

应用

• Ultrafiltration with 10,000 Dalton molecular weight cutoff Ultracel^® membrane
• Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
• Purification of macromolecular components found in tissue culture extracts or cell lysates, primer removal, linkers, or macromolecular labels from a reaction mix, and protein removal prior to HPLC
• Desalting, buffer exchange, and protein dialysis

特点和优势

• Ultracel^® regenerated cellulose membrane with 10 kDa NMWCO delivers retentate recovery of greater than 90%
• Concentrates 500 μL down to 15 μL
• Concentration factor 25x to 30x
• High sample recovery (greater than 90% of dilute starting solution)
• Fast processing time: 10 to 30 minutes
• Reverse spin capability provides consistent recoveries

外形

色码：透明

法律信息

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

基本信息

产品性质