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Monoclonal Anti-phospho-β-Catenin (pThr41) antibody produced in mouse

品牌
Sigma-Aldrich
货号
C8616
规格纯度
clone BCT-41, purified immunoglobulin, buffered aqueous solution
参考价格
4731.57 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Monoclonal Anti-phospho-β-Catenin (pThr41) (mouse IgG1 isotype) is derived from the BCT-41 hybridoma produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice. β-catenin belongs to the armadillo family and comprises the N-terminal region with 12 imperfect repeats and a conserved C-terminal region. The locus 3p22.1 in the human chromosome harbors β-catenin gene.

特异性

Monoclonal Anti-phospho-β-Catenin (pThr41) reacts specifically with human β-catenin phosphorylated at Thr41 (~ 94 kDa).

免疫原

synthetic phosphopeptide corresponding to amino acids 38-50 (pThr41) of human β-catenin, conjugated to KLH.

应用

Monoclonal Anti-phospho-β-Catenin (pThr41) antibody produced in mouse may be used in:

  • enzyme-linked immunosorbent assay (ELISA)
  • immunocytochemistry
  • immunoblotting
  • immunoprecipitation

生化/生理作用

β-catenin is a prime component of the wingless (Wnt) pathway and plays a key role in gene expression during development. β-Catenin activity is regulated by phosphorylation on serine/threonine residues located at the protein amino terminus.. The phosphorylation of β-catenin occurs in a protein complex that includes the APC complex (Adenomatous Polyposis Coli), together with Axin by casein kinase I (CKI) and glycogen synthase kinase-3 (GSK-3). The phosphorylation of the β-catenin protein targets the protein to degradation by the β-TrCP (β-transducin repeat-containing protein). Mutations in the serine and threonine residues in the amino-terminal region of the protein and in particular in the phosphorylation sites in that region, cause a reduction in the degradation of the protein and can lead to cancer. Monoclonal antibodies to phospho- β-catenin (pThr41) are an important tool for studying the regulation of β-catenin by phosphorylation and thus the transcription control of many proteins

外形

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

储存及稳定性

For continuous use, store at 2-8 ℃ for up to one month. For prolonged storage, freeze in working aliquots. Repeated freezing and thawing, or storage in frost-free freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号MFCD07785413

产品性质

质量水平200
生物来源mouse
偶联物unconjugated
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆BCT-41, monoclonal
形式buffered aqueous solution
分子量antigen ~94 kDa
species reactivityhuman
浓度5-10 mg/mL (IgG fraction of antiserum)
technique(s)immunocytochemistry: 1:50 using 293 T (human embryonic kidney cell)
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 8-10 μg/mL using extract of cultured human 293T cells treated with Lici (20 mM) and calyculin A (50 nM)
同位素/亚型IgG1
UniProt登记号P35222
运输dry ice
储存温度−20℃
Gene Informationhuman ... CTNNB1(1499)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKnwg
闪点(F)Not applicable
闪点(C)Not applicable

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