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Cholesterol Oxidase microbial

品牌
Sigma-Aldrich
CAS
9028-76-6
货号
C1235
规格纯度
recombinant, lyophilized powder, ≥10 units/mg protein
参考价格
2863.39 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

应用

Cholesterol oxidase is used to determine serum cholesterol. The enzyme also finds application in the microanalysis of steroids in food samples and in distinguishing 3-ketosteroids from 3b-hydroxysteroids. Transgenic plants expressing cholesterol oxidase are being investigated in the fight against the cotton boll weevil. CHOD has also been used as a molecular probe to elucidate cellular membrane structures.

包装

100 units in glass bottle

生化/生理作用

Cholesterol oxidase (CHOD) is a monomeric flavoprotein containing FAD that catalyzes the first step in cholesterol catabolism. This bifunctional enzyme oxidizes cholesterol to cholest-5-en-3-one in an FAD-requiring step. This is subsequently isomerized to cholest-4-en-3-one with the release of H2O2. Optimum pH of the enzyme is 7.0. Hg2+, Ag+, ionic detergents inhibit the enzyme activity.

单位定义

One unit will convert 1.0 μmol of cholesterol to 4-cholesten-3-one per minute at 37 ℃ and pH 7.0 in a peroxidase linked system.

制备说明

Dissolves in cold 50 mM potassium phosphate buffer, pH 7.0. Solution is to be prepared just before use.

基本信息

酶学委员会(EC)编号1.1.3.6 ( BRENDA | IUBMB )
EC 号232-842-1
MDL编号MFCD00130783
NACRESNA.54

产品性质

质量水平200
形式lyophilized powder
specific activity≥10 units/mg protein
分子量 55 kDa
溶解性50 mM potassium phosphate buffer, pH 7.0: soluble
储存温度−20℃

安全信息

储存分类代码13 - Non Combustible Solids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable
个人防护装备Eyeshields, Gloves, type N95 (US)

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