产品说明
一般描述
Cytidine monophosphate (CMP)-Sialic Acid Synthetase from Neisseria meningitidis group B is encoded in neuA gene. The protein has a molecular weight of 24.8 kDa.
应用
The enzyme has been utilized to synthesize CMP-sialic acid and its derivatives.
生化/生理作用
Cytidine monophosphate (CMP)-sialic acid synthetase catalyses the conversion of N?acetylneuraminic acid (NeuNAc) to CMP-NeuNAc. CMP-sialic acid synthetase has globular α/β domain and is categorised under αβα three-layered sandwich fold. The dimerization domain aids the interaction between the monomers. It also has mononucleotide binding and NeuAc binding pocket. Mg2+ is essential for the catalytic functionality of CMP-sialic acid synthetase.
单位定义
One unit will catalyze the formation of 1 μmol CMP-Neu-5-Ac from Neu-5-Ac and CTP per minute at 37 ℃ at pH 8.0.
外形
Supplied as a lyophilized powder containing Tris-HCl and NaCl.
分析说明
Enzymatic activity assays are performed in Tris-HCl buffer (100 mM, pH 8.5) containing Neu-5-Ac (1 mM) and CTP (1 mM) at 37 ℃ for 30 min and analyzed using capillary electrophoresis with a UV detector (200 nm).
基本信息
| 酶学委员会(EC)编号 | 2.7.7.43 ( BRENDA | IUBMB ) |
| MDL编号 | MFCD01323074 |
| NACRES | NA.54 |
产品性质
| 质量水平 | 200 |
| 重组 | expressed in E. coli BL21 |
| 形式 | lyophilized solid |
| specific activity | ≥10 units/mg protein |
| 分子量 | 26.0 kDa |
| 运输 | dry ice |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
