产品说明
一般描述
IgG is a monomeric immunoglobulin, consists of two heavy chains (gamma chains) and two light chains. Each molecule has two antigen binding sites. IgG is the most abundant immunoglobulin. It is approximately equally distributed in blood and in interstitial fluid, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, providing protection to the fetus in its first weeks of life before its own immune system has developed. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).
特异性
The antibody reacts with the heavy chains of human IgG and with the light chains common to most human immunoglobulins.
免疫原
Prepared from purified human IgG.
应用
Research Category
Secondary & Control Antibodies
EIA: 1:20,000-1:400,000 using enzyme-streptavidin conjugate.
Western blots: 1:20,000-1:400,000 using enzyme-streptavidin conjugate.
Immunohistochemistry: 1:500-1:5,000 using enzyme-streptavidin conjugate.
Flow cytometry: 1:200-1:1,000.
Fluorescence Immunohisto/cytochemistry: 1:200-1:1,1000.
Optimal working dilutions must be determined by the end user.
Research Sub Category
Whole Immunoglobulin Secondary Antibodies
This Goat anti-Human IgG Antibody, biotin conjugate is validated for use in ELISA, WB for the detection of Human IgG.
外形
Purified by immunoaffinity chromatography using antigen coupled to agarose beads. Lyophilized. Buffer is 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA, and 0.05% sodium azide.
ImmunoAffinity Purified
储存及稳定性
Maintain lyophilized product at 2°-8°C for up to 12 months from date of receipt. After reconstitution the product is stable for six weeks at 2°-8°C as an undiluted liquid. Reconstitute vial with 2 mL of distilled water. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of antibody (and buffer salts) will decrease to one-half of the original after the addition of glycerol and final working dilutions must be adjusted accordingly. Avoid repeated freeze thaw cycles.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.46 |
产品性质
| 质量水平 | 100 |
| 生物来源 | goat |
| 偶联物 | biotin conjugate |
| 抗体形式 | affinity purified immunoglobulin |
| antibody product type | secondary antibodies |
| 克隆 | polyclonal |
| species reactivity | human |
| manufacturer/tradename | Chemicon® |
| technique(s) | ELISA: suitable western blot: suitable |
| 运输 | wet ice |
安全信息
| 象形图 |  |
| 警示用语： | Warning |
| 危险声明 | H312 + H332 - H412 |
| 预防措施声明 | P261 - P271 - P273 - P280 - P302 + P352 + P312 - P304 + P340 + P312 |
| 危险分类 | Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 3 |
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 3 |
