产品说明
一般描述
BAFF (B lymphocyte activating factor) is a member of the tumor necrosis factor (TNF) ligand family which is expressed in T Cells, macrophages, monocytes and dendritic cells. It is also known as BLyS, THANK, TALL, zTNF4 and TNFS20. BAFF enhances B cell survival in vitro and has emerged as a key regulator of peripheric B cell and it is vital homeostatic cytokine for B cells that helps regulate both innate and adaptive immune responses. BAFF binds to three TNF receptors: B-cell maturation antigen (BCMA/TNFRSF17), transmembrane activator and calcium modulator and cyclophilin ligand interactor(TACI/TNFRSF13B) and BAFF receptor (BAFF R/BR3/TNFRSF 13C).The human BAFF gene code for a 285 amino acids type II transmembrane protein. Recombinant human soluble BAFF is a 151 amino acids containing the TNF-like portion of the extracellular domain of BAFF.
外形
Lyophilized from a 20 mM PBS buffer at pH 7 and 0.2 M NaCl.
重悬
Lyophilized protein should be reconstituted in water to a concentration of 25 - 50 ng /μl. Due to the protein nature, dimmers and multimers may be observed.
分析说明
The activity is determined by dose-dependeant stimulation of proliferation B cell from Human PBMC. Cell proliferation was measured by MTT method.
*activity results may vary with PBMC donors.
ED50 ≤ 50 ng/mL
其他说明
Extinction Coefficient: E0.1% = 0.791 (A280 nm)
p.I: 6.03
基本信息
| NACRES | NA.32 |
产品性质
| 质量水平 | 200 |
| 生物来源 | human |
| 重组 | expressed in Nicotiana |
| 测定 | >97% (SDS-PAGE) |
| 形式 | lyophilized |
| 效能 | ≤50 ng/mL ED50 |
| 分子量 | 18-20 kDa (a glycosilated polypeptide chain containing 151 amino acids) |
| 包装 | pkg of 10 μg |
| 储存条件 | avoid repeated freeze/thaw cycles |
| 杂质 | Endotoxin, tested (LAL test, < 0.04 EU/ ug protein) |
| UniProt登记号 | Q9Y275 |
| 储存温度 | −20℃ |
| Gene Information | human ... TNFSF13B(10673) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
