产品说明

一般描述

Recombinant murine TNF-α is produced in E. coli and purified by standard chromatographic techniques.
Contents:
5 μg/ml solution in PBS and 1 mg/ml BSA, filtered through a 0.2 μm pore size membrane

特异性

Mouse mTNF-α is effective on mouse, rat, rabbit, and human cells.
Specific activity/EC₅₀: >4 x 108 U/mg, <0.0025 ng/ml (mTNF-a, NIBSC, interim standard, 88/532), at least the same specific activity (EC₅₀) compared to the indicated standard is guaranteed.

应用

TNF-α causes selective necrosis of murine tumors when injected into tumor-bearing mice. In vitro, human TNF-α has direct cytolytic or cytostatic activity on certain transformed cells. In this context, it acts synergistically with interferon-γ. It has also been used for cell stimulation studies.

质量

Purity: >95% (SDS-PAGE)
Endotoxin level: <0.1 EU/μg (LAL-test), <10 EU/ml (LAL-test)
Note: 1 EU corresponds to 0.1 ng

产品规格

Primary structure: One polypeptide chain (156 amino acids) identical to natural mTNF-a, but not glycosylated. Glycosylation is not essential for biological activity.

序列

Chain Length 156 AA
One polypeptide chain (156 amino acids) identical to natural mTNF-α, but not glycosylated. Glycosylation is not essential for biological activity.

单位定义

EC₅₀ definition: The amount of mTNF-α that is required to mediate half-maximal cytotoxicity (MTT cleavage) with WEHI 164 cells in the presence of actinomycin D (1 unit equals ≤0.0025 ng/ml).

制备说明

Storage conditions (working solution): -15 to -25 ℃
The solution can be stored in aliquots at -15 to -25 ℃.
Note: Avoid repeated freezing and thawing.

其他说明

仅用于生命科学研究。不可用于诊断。

产品性质

安全信息