产品说明

一般描述

MTP^™ Taq DNA Polymerase is a recombinant thermostable enzyme from Thermus aquaticus expressed in E. coli and purified using a proprietary process to minimize levels of contaminating DNA. The enzyme has both 5′→3′ DNA polymerase and exonuclease activities, is ∼95 kDa by SDS-PAGE, and has no detectable endonuclease or 3′→5′ exonuclease activities. Each lot of MTP Taq undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA.

Contaminating DNA present in most other polymerase preparations often preclude or obscure the accurate interpretation of results, especially when targeting conserved sequences, e.g., bacterial 16S rRNA region. Through Sigma′s proprietary DNA removal methods and strict quality control standards, we can ensure the absence of the most commonly found contaminant DNA. Each lot of MTP Taq is assayed using PCR and primers specific to the conserved region of bacterial 16S rRNA, the Taq expression vector, and the human β-actin gene.

While MTP Taq ensures a high-quality, low contaminant DNA polymerase for reliable PCR amplification, DNA contaminants can be introduced into PCR through a number of other reagents. To further minimize the risk of contaminant DNA during PCR, we include 10× MTP Taq Buffer with each tube of MTP Taq DNA Polymerase. Each lot of 10× MTP Taq Buffer undergoes the same strict quality control testing as MTP Taq DNA polymerase to ensure the absence of contaminating DNA. To prevent false positive PCR results, only DNA-free reagents should be used in PCR reactions with MTP Taq DNA polymerase.

应用

MTP^™ Taq DNA Polymerase has been used:

• for the amplification of bacterial 16S rRNA genes from purified DNA
• bacterial genome analysis
• pathogen detection

生化/生理作用

MTP^™ Taq polymerase catalyzes oligonucleotide primer-driven, DNA template dependent incorporation of dNTPs into complimentary DNA strands.

特点和优势

• Low contaminant DNA polymerase
• Prevents false positive PCR results from contaminating bacterial DNA

组分

• MTP Taq DNA聚合酶(D7067)
• 10x MTP Taq缓冲液(M9943)

单位定义

一个酶活性单位是指在74 ℃下，在30分钟内将10 nmol 脱氧核糖核苷三磷酸掺入酸性可沉淀DNA中所需的酶量。

其他说明

储存在-20℃。为方便起见，10x MTP Taq 缓冲液可以在室温下储存。
访问www.sigma-aldrich.com/specialtyenzymes，查看关于特色酶产品的更多信息。
访问www.sigma-aldrich.com/mtptaq，查看有关MTP Taq DNA聚合酶的更多详细信息。

法律信息

No license is conveyed with the purchase of this product under any of US Patents Nos. 5,804,375, 5,994,056, 6,171,785, 6,214,979, 5,538,848, 5,723,591, 5,876,930, 6,030,787, and 6,258,569, and corresponding patents outside the United States, or any other patents or patent applications, relating to the 5′ Nuclease and dsDNA-Binding Dye Processes. For further information contact the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
MTP is a trademark of sigma-aldrich Co. LLC

基本信息

产品性质

安全信息