产品说明
一般描述
RAOSMC provide an excellent model system to study all aspects of cardiovascular function and disease, especially those related to mechanisms of hyperplasia and hypertrophy of intimal smooth muscle cells leading to vascular occlusion in atherosclerosis and stent restenosis.
RAOSMC from Cell Applications, Inc. have been utilized in dozens of research studies to:
- Provide a gold standard control for smooth muscle markers ?-SMA, calponin and caldesmon expression (Imamura, 2010)
- Elucidate cytokines and growth factors signaling pathways implicated in the molecular regulation of smooth muscle cell proliferation, migration, and overall vascular function (Alexis, 2009; Liu, 2009; Tan, 2009; Ko, 2012; Malabanan, 2012; Santiago, 2012; Newaz, 2013); and demonstrate that peach extract is able to inhibit Ang II-induced intracellular Ca2+ elevation and the generation of ROS, thus showing promise in prevention of cardiovascular diseases (Kono, 2013)
- Investigate extracellular matrix properties and its role in cardiovascular health, repair of damaged vasculature and successful tissue engineering (Chiang, 2009; Kothapalli, 2009a,b,c)
- Demonstrate that hyperglycemia-induced elastin degradation products lead to vascular smooth muscle cell calcification and identify periostin, also implicated in mechanical stress and inflammation responses, as an early marker for this process (Shanmugam, 2013; Sinha, 2013; Yamashita, 2013)
- Develop advanced stent technology, including novel surface materials, and drug delivery systems to prevent restenosis and other occlusive vasculopathies (Taite, 2011; Midwinter, 2012; Li, 2013)
Characterization: positive for smooth muscle cell specific alpha-actin expression.
细胞系来源
Aorta
应用
Aortic wall contraction, role of smc under normal conditions, blood pressure, distribution of oxygenated blood through systemic circulation.
组分
Rat Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO
质量
Each lot was tested for proper morphology, Population Doublings, Negative for HIV, Hepatitis B, Hepatitis C, mycoplasma, bacteria, and fungi.
制备说明
- 2nd passage, >500,000 cells in Rat Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO
- Can be cultured at least 16 doublings
传代培养常规
Please refer to the RAOSMC Culture Protocol.
基本信息
| NACRES | NA.81 |
产品性质
| 生物来源 | rat aorta (healthy adult tunica intima and media) |
| 包装 | pkg of 500,000 cells |
| 生长模式 | Adherent |
| 形态学 | smooth muscle |
| technique(s) | cell culture | mammalian: suitable |
| 相关疾病 | cardiovascular diseases |
| 运输 | dry ice |
| 储存温度 | −196℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
