位置:首页 > 品牌 > Sigma-Aldrich >

Human Aortic Endothelial Cells: HAOEC: Pre-Screened for Angiogenesis &VEGF signaling, adult

品牌
Sigma-Aldrich
货号
S304-05A
包装型号
规格纯度
参考价格
14177 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Endothelial cells (EC) line the blood vessels of the entire circulatory system, from aorta to capillaries, and act as a barrier between circulating blood and the rest of the vessel wall/surrounding tissues. Endothelial cells are involved in the processes of angiogenesis, vasculogenesis, vasoregulation, coagulation, fibrinolysis and selective blood barrier. They are also implicated in pathophysiological processes, including cardiovascular disease and cancer development.

Matched smooth muscle cells obtained from the same arteries are available as well.The most commonly used human endothelial cells are Human Umbilical Vein Endothelial Cells, (HUVEC), Human Microvascular Endothelial Cells (HMVEC) and Human Aortic Endothelial Cells (HAOEC). Select lots of HUVEC, HMVEC, and HAOEC have been pre-screened to demonstrate stimulation-dependent angiogenesis and key endothelial cell signaling pathways (phosphorylation of VEGFR, Akt, MAPK, and expression of Tie2, eNOS, Axl and Etk/Bmx.

VEGF-Stimulated Signaling in Pre-Screened Endothelial Cells
VEGF receptor-2 (VEGFR-2) is a major VEGF of endothelial cells. VEGFR-2-mediated signaling plays a critical role in angiogenesis, including regulation of proliferation, differentiation, cell movement, and survival of endothelial cells. VEGF-induced receptor dimerization triggers activation of VEGFR-2 tyrosine kinase and autophosphorylation at a specific set of tyrosine residues, which serve as docking sites for downstream signaling components leading to activation of downstream signaling molecules, including phosphorylation of Akt and p44/42-MAPK.

Expression of Signaling Biomarkers in Pre-Screened Endothelial Cells
Prescreened Endothelial Cells express the following important biomarkers:

  • Tie2, a receptor tyrosine kinase critical for the angiogenic remodeling, sprout formation, survival of endothelial cells and vessel stabilization processes;
  • eNOS (endothelial Nitric Oxide Synthase), the enzyme that producse NO which is an important signaling molecule that regulates a diverse range of physiological events and is required for normal endothelial function;
  • Axl, a receptor tyrosine kinase whose ligand is the survival factor Gas6 (growth arrest-specific gene 6 product) implicated in many processes, such as cell survival, leukocyte transmigration and neointima formation.
  • Etk/Bmx (Endothelial/epithelial Tyrosine Kinase), a member of the Btk family, participates in signal transduction stimulated by growth factor receptors, cytokine receptors, G-protein-coupled receptors, antigen receptors, and integrins, and has been implicated in cell adhesion, migration, proliferation, and survival.

细胞系来源

Blood Vessels

应用

cardiovascular function, studies on immune system and graft rejection, development of 3D endothelialized engineered tissues, drug discovery, stent-graft compatibility testing

组分

Basal Medium containing 10% FBS & 10% DMSO

制备说明

  • 2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
  • Can be cultured at least 16 doublings

传代培养常规

Please refer to the HAOEC Culture Protocol.

基本信息

NACRESNA.81

产品性质

质量水平100
生物来源human blood vessels
包装pkg of 500,000 cells
生长模式Adherent
核型2n = 46
形态学Endothelial
technique(s)cell culture | mammalian: suitable
相关疾病diabetes; cardiovascular diseases
运输dry ice
储存温度−196℃

安全信息

储存分类代码13 - Non Combustible Solids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

推荐产品
| 首页 | 联系我们 | 会员服务 | 广告服务 | 友情链接 |
版权所有 CopyRight © 2008-2024 粤ICP备08119708号