产品说明
一般描述
RBMVEC from Cell Applications, Inc. provide an excellent model system to study many aspects of endothelial function and disease, especially those related to the blood-brain barrier (BBB), including interactions between neurons, astorcytes and endothelial cells, brain cognitive function, search for therapeutic modulators, and develop novel drug delivery methods for crossing the BBB.
RBMVEC from Cell Applications, Inc. have been utilized in a number of research publications, for example to:
- Serve as a gold standard control for endothelial markers VE-cadherin and CD31 expression (Johansson, 2009)
- Demonstrate a better brain penetration of cardiovascular and anti-stroke drugs Tanshinones IIA and IIB and Cryptotanshinone in the presence of PgP or MRP1/2 inhibitors (Chen, 2007; Yu, 2007; Zhi-Wei, 2007) or by using PEGylated gold nanoparticles (Etame, 2011)
- Show that removal of excess glutamate from the blood by glutamate oxaloacetate transaminase correlates to a decrease in brain glutamate levels and confers neuroprotection against ischemic stroke (Campos, 2011)
- Assess, along with Rat Astrocytes (RA) also obtained from Cell Applications, Inc., neuroprotective capabilities of bioenergy stabilizers in an in vitro model of stroke (Liu, 2013)
- Show that bile acids cause activation of Rac1 and phosphorylation of occluding, explaining increased permeability of the blood brain barrier seen during obstructive cholestasis (Quinn, 2014)
- Demonstrate that saquinavir-associated dementia in HIV-positive patients is exacerbated by smoking due to the BBB disruptive effects of both nicotine and saquinavir, mediated by decrease in Notch-4 expression and increase in ROS (Manda, 2010)
- Show that treatment with connexin43 mimetic peptide which transiently blocks gap junction function, reduces vascular leak and can be used to treat of central nervous system ischaemia (Danesh-Meyer, 2012)
- Demonstrate that oxygen and glucose deprivation increase ROS, cytochrome c levels and caspase-3 activity, induce tight junction disruption and actin stress fiber formation, leading to BBB break down (Alluri, 2013)
- Investigate the interactions between neurons, astorcytes and endothelial cells by showing that exposure to metabolic stress induces tissue-type plasminogen activator release from neurons which induces AMPK activation, membrane recruitment of GLUT1, and GLUT1-mediated glucose uptake in astrocytes and endothelial cells, followed by the synthesis and release of lactic acid from astrocytes, and that the uptake of this lactic acid via the monocarboxylate transporter-2 promotes survival in neurons (An, 2013)
- Study the proapoptotic and anti-angiogenic role of p75NTR in choroidal neovascularization (Tahiri, 2013)
细胞系来源
Brain
应用
Endothelial vessel function, cell signaling, angiogenesis.
组分
Rat Brain Endothelial Cell Basal Medium that contains 10% FBS and 10% DMSO
质量
Each lot was tested for proper morphology, Population Doublings, Negative for HIV, Hepatitis B, Hepatitis C, mycoplasma, bacteria, and fungi.
制备说明
- 2nd passage, >500,000 cells in Rat Brain Endothelial Cell Basal Medium that contains 10% FBS and 10% DMSO
- Can be cultured at least 16 population
传代培养常规
Please refer to the RBMVEC Culture Protocol.
基本信息
NACRES | NA.81 |
产品性质
质量水平 | 100 |
生物来源 | rat brain (Sprague Dawley, adult) |
包装 | pkg of 500,000 cells |
生长模式 | Adherent |
形态学 | Endothelial |
technique(s) | cell culture | mammalian: suitable |
运输 | dry ice |
储存温度 | −196℃ |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 3 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |