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Rat Brain Microvascular Endothelial Cells: RBMVEC, adult

品牌
Sigma-Aldrich
货号
R840-05A
包装型号
规格纯度
参考价格
13965.1 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

RBMVEC from Cell Applications, Inc. provide an excellent model system to study many aspects of endothelial function and disease, especially those related to the blood-brain barrier (BBB), including interactions between neurons, astorcytes and endothelial cells, brain cognitive function, search for therapeutic modulators, and develop novel drug delivery methods for crossing the BBB.

RBMVEC from Cell Applications, Inc. have been utilized in a number of research publications, for example to:

  • Serve as a gold standard control for endothelial markers VE-cadherin and CD31 expression (Johansson, 2009)
  • Demonstrate a better brain penetration of cardiovascular and anti-stroke drugs Tanshinones IIA and IIB and Cryptotanshinone in the presence of PgP or MRP1/2 inhibitors (Chen, 2007; Yu, 2007; Zhi-Wei, 2007) or by using PEGylated gold nanoparticles (Etame, 2011)
  • Show that removal of excess glutamate from the blood by glutamate oxaloacetate transaminase correlates to a decrease in brain glutamate levels and confers neuroprotection against ischemic stroke (Campos, 2011)
  • Assess, along with Rat Astrocytes (RA) also obtained from Cell Applications, Inc., neuroprotective capabilities of bioenergy stabilizers in an in vitro model of stroke (Liu, 2013)
  • Show that bile acids cause activation of Rac1 and phosphorylation of occluding, explaining increased permeability of the blood brain barrier seen during obstructive cholestasis (Quinn, 2014)
  • Demonstrate that saquinavir-associated dementia in HIV-positive patients is exacerbated by smoking due to the BBB disruptive effects of both nicotine and saquinavir, mediated by decrease in Notch-4 expression and increase in ROS (Manda, 2010)
  • Show that treatment with connexin43 mimetic peptide which transiently blocks gap junction function, reduces vascular leak and can be used to treat of central nervous system ischaemia (Danesh-Meyer, 2012)
  • Demonstrate that oxygen and glucose deprivation increase ROS, cytochrome c levels and caspase-3 activity, induce tight junction disruption and actin stress fiber formation, leading to BBB break down (Alluri, 2013)
  • Investigate the interactions between neurons, astorcytes and endothelial cells by showing that exposure to metabolic stress induces tissue-type plasminogen activator release from neurons which induces AMPK activation, membrane recruitment of GLUT1, and GLUT1-mediated glucose uptake in astrocytes and endothelial cells, followed by the synthesis and release of lactic acid from astrocytes, and that the uptake of this lactic acid via the monocarboxylate transporter-2 promotes survival in neurons (An, 2013)
  • Study the proapoptotic and anti-angiogenic role of p75NTR in choroidal neovascularization (Tahiri, 2013)

细胞系来源

Brain

应用

Endothelial vessel function, cell signaling, angiogenesis.

组分

Rat Brain Endothelial Cell Basal Medium that contains 10% FBS and 10% DMSO

质量

Each lot was tested for proper morphology, Population Doublings, Negative for HIV, Hepatitis B, Hepatitis C, mycoplasma, bacteria, and fungi.

制备说明

  • 2nd passage, >500,000 cells in Rat Brain Endothelial Cell Basal Medium that contains 10% FBS and 10% DMSO
  • Can be cultured at least 16 population

传代培养常规

Please refer to the RBMVEC Culture Protocol.

基本信息

NACRESNA.81

产品性质

质量水平100
生物来源rat brain (Sprague Dawley, adult)
包装pkg of 500,000 cells
生长模式Adherent
形态学Endothelial
technique(s)cell culture | mammalian: suitable
运输dry ice
储存温度−196℃

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable

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