位置:首页 > 品牌 > Sigma-Aldrich >

Anti-Aurora-A Kinase antibody, Mouse monoclonal

Monoclonal Anti-Aurora-A Kinase 小鼠抗

品牌
Sigma-Aldrich
货号
A1231
规格纯度
clone 35C1, purified from hybridoma cell culture
参考价格
5809.73 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Anti-Aurora-A Kinase antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the hybridoma 35C1 produced by the fusion of mouse myeloma cells (SP2/0-Ag14) and splenocytes from BALB/c mice immunized with recombinant human Aurora-A Kinase. Aurora-A Kinase is encoded by serine/threonine kinase (STK15) gene. During S-phase to mitosis progression in cell cycle, the protein is located at the centrosome.

特异性

Monoclonal Anti-Aurora-A Kinase antibody is specific for Aurora-A Kinase in humans and mice.

免疫原

recombinant human Aurora-A kinase.

应用

Monoclonal Anti-Aurora-A Kinase antibody is suitable for use in immunocytochemistry, immunoprecipitation, immunoblot, indirect ELISA and western blot (0.25-0.5 μg/mL using HeLa total cell extract). The antibody product does not inhibit kinase activity of Aurora-A kinase, hence the product can be used to measure the in vivo aurora-A kinase activity after immunoprecipitation.
Anti-Aurora-A Kinase antibody, Mouse monoclonal has been used in immunohistochemistry.

生化/生理作用

Aurora-A (Aur-A) kinase is a serine threonine kinase that regulates cell division. Elevated levels of Aur-A have been associated with inhibition of apoptosis, and evasion of spindle assembly checkpoints. Aur-A may also be used as a prognostic marker for nasopharyngeal cancers in humans .
Aurora-A kinase is involved in centrosome separation, centrosome maturation, bipolar spindle assembly and stability. Monopolar spindles are formed in the cell when the expression of aAurora-A kinase is inhibited by an appropriate siRNA, while over-expression of this protein results in centrosome amplification and polyploidy of the cell because of cytokinesis failure. Aurora-A activity is essential for the recruitment of cyclin dependent kinase (CDK)-cyclin B1 to the centrosome, which relates with its activation and the commitment of the cells to mitosis. Activation of CDK1 kinase is by dephosphorylation of Tyr15 by CDC25B. Aurora-A kinase phosphorylates CDC25B both in vitro and in vivo at Ser353. This phosphorylation occurs at the centrosome during progression from prophase to anaphase. This regulation is important for entry into mitosis.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号MFCD06411780
eCl@ss32160410
NACRESNA.44

产品性质

质量水平200
生物来源mouse
重组expressed in mouse cell line
偶联物unconjugated
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆35C1, monoclonal
形式PBS solution
分子量antigen ~46 kDa
species reactivitymouse, human
浓度~2 mg/mL
technique(s)immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 0.25-0.5 μg/mL using HeLa total cell extract
同位素/亚型IgG2b
UniProt登记号O14965
运输dry ice
储存温度−20℃
Gene Informationhuman ... AURKA(6790), AURKA(6790)
mouse ... Aurka(20878), Aurka(20878)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable
个人防护装备Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Sigma-Aldrich

推荐产品
| 首页 | 联系我们 | 会员服务 | 广告服务 | 友情链接 |
版权所有 CopyRight © 2008-2024 粤ICP备08119708号