产品说明
一般描述
Monoclonal Anti-ATM (mouse IgG1 isotype) is derived from the hybridoma MAT3-4G10/8 produced by the fusion of mouse myeloma cells (NSO) and splenocytes from BALB/c mice immunized with a peptide.
特异性
Monoclonal Anti-ATM antibody reacts specifically with mouse and human ATM.
免疫原
peptide spanning positions 1967-1988 of mouse ATM containing a cysteine at its NH2 terminus coupled to KLH.
应用
Monoclonal Anti-ATM antibody can be used in immunoblotting , ELISA and immunoprecipitation.
生化/生理作用
Anti-Ataxia-Telangiectasia Mutated (ATM) is responsible for the activation and stabilization of p53 in response to double-strand break (DSB). ATM phosphorylates p53 directly on Ser15 and concomitantly activates other kinases that phosphorylate the same molecule on additional sites. Furthermore, human homolog of double minute 2 (Hdm2) is phosphorylated by ATM on Ser395 and this phosphorylation inhibits Hdm2-mediated degradation of p53.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD01321740 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 重组 | expressed in mouse cell line |
| 偶联物 | unconjugated |
| 抗体形式 | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| 克隆 | MAT3-4G10/8, monoclonal |
| 形式 | PBS solution |
| 分子量 | antigen ~300 kDa |
| species reactivity | mouse, human |
| 包装 | antibody small pack of 25 μL |
| 浓度 | ~2 mg/mL |
| technique(s) | immunoprecipitation (IP): suitable indirect ELISA: suitable western blot: 0.1-0.2 μg/mL using HEK-293T total cel extract |
| 同位素/亚型 | IgG1 |
| UniProt登记号 | Q13315 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... ATM(472) mouse ... Atm(11920) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
