产品说明
应用
Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation . Product A2230 is a high activity apyrase.
在多种马铃薯普通栽培种中至少发现了两种同工酶：一种具有高 ATP 酶/ADP 酶比率(约 10)，另一种该比率较低(约 1)。
反应：ATP → ADP+Pi → AMP+2Pi。
包装
100 units in glass bottle
按 ATP 酶单位销售。
生化/生理作用
Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis . Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM.
单位定义
One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 ℃.
外形
Lyophilized powder containing potassium succinate buffer salts.
制备说明
来源于红皮土豆
基本信息
| 酶学委员会(EC)编号 | 3.6.1.5 ( BRENDA | IUBMB ) |
| MDL编号 | MFCD00130542 |
| NACRES | NA.54 |
产品性质
| 质量水平 | 300 |
| 形式 | lyophilized powder |
| 质量 | High Activity |
| ATPase activity | ≥600 units/mg protein |
| secondary activity | ≥50 % of base activity ADPase |
| 组成 | protein, ≥30% |
| 异质活性 | Acid Phosphatase ≤2% of base activity |
| 运输 | wet ice |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
