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Cas9-RFP Lenti Plasmid

品牌
Sigma-Aldrich
货号
CAS9RFPVP
包装型号
规格纯度
参考价格
2486 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

This product is a lentiviral plasmid that utilizes the EF1 alpha promoter to drive expression of Cas9 and RFP linked by a 2A peptide (EF1a-Cas9-2A-RFP) allowing for easy visualization of successful transfection or transduction. Use Sigma′s lentiviral Cas9 plasmid for generation of lentiviral particles and efficient production of stable cell lines expressing Cas9 for CRISPR based genome editing. Sigma′s Cas9-RFP lenti plasmid is one part of a two part CRISPR system with individual Cas9 and gRNA expression vectors. For easy fluorescence microscopy a MOI greater than 200 should be employed when packaged into lentiviral particles.

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应用

Functional Genomics/Target Validation

  • Creation of gene knockouts in multiple cell lines
  • Complete knockout of genes not amenable to RNAi
  • Manufacture of Cas9-RFP expressing Lentiviral Particles

特点和优势

  • Highly specific
  • Highly active
  • Ready to use purified plasmid DNA

原理

CRISPR/Cas systems are employed by bacteria and archaea as a defense against invading viruses and plasmids. Recently, the type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a single gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB.

法律信息

CRISPR Use License Agreement

Lentiviral, WPRE and Evrogen Fluorophore Licenses

产品性质

重组expressed in E. coli
包装vial of 50 μL
浓度20 ng/μL in TE buffer; DNA (1μg of Plasmid DNA)
application(s)CRISPR
报告基因RFP
selectionampicillin
运输dry ice
储存温度−20℃

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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