产品说明
一般描述
Anti-CAM 5.2 is a mouse monoclonal antibody that was generated by using the human colorectal carcinoma cell line HT24. Anti-CAM 5.2 is not technically a pan-keratin antibody because its reactivity has been reported to be restricted primarily to a few keratins. However, since the few keratins are widely expressed in formalin-fixed, paraffin-embedded tissues, especially in simple and glandular epithelia, this antibody can be successfully used as a reliable marker for neoplasms of epithelial differentiation. Anti-CAM 5.2 reacts with the majority of epithelial tumors, including lung, liver, pancreas, GI tract, breast, genitourinary system, female reproductive organs and some endocrine organs.Hepatocellular carcinoma is targeted by anti-CAM 5.2, but not by pan-cytokeratin AE1/AE3 antibody.1 Adrenal cortical carcinomas frequently do not react with anti-CAM 5.2.Sebaceous carcinoma, squamous cell carcinoma, and basal cell carcinoma may show an overlapping morphology. Anti-CAM 5.2 is useful to discriminate among them. Literature indicates no reactivity by anti-CAM 5.2 for squamous cell carcinoma while sebaceous carcinoma and basal cell carcinoma show 73% and 44% immunoreactivity respectively.Similar to other broad-spectrum keratin antibodies, such as the AE1/AE3 antibody cocktail, anti-CAM 5.2 may stain astrocytic tumors, but the positivity reported for this tumor cross-reactivity is lower than with anti-AE1/AE3.
联系
Cytokeratin (CAM 5.2) Positive Control Slides, Product No. 452S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).
外形
Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide
其他说明
For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com
法律信息
Cell Marque is a trademark of sigma-aldrich Co. LLC
基本信息
| NACRES | NA.41 |
产品性质
| 质量水平 | 500 100 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | culture supernatant |
| antibody product type | primary antibodies |
| 克隆 | CAM 5.2, monoclonal |
| 描述 | For In Vitro Diagnostic Use in Select Regions (See Chart) |
| 形式 | buffered aqueous solution |
| species reactivity | human |
| 包装 | vial of 0.1 mL concentrate (452M-94) vial of 0.5 mL concentrate (452M-95) bottle of 1.0 mL predilute (452M-97) vial of 1.0 mL concentrate (452M-96) bottle of 7.0 mL predilute (452M-98) |
| manufacturer/tradename | Cell Marque™ |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:25-1:100 |
| 同位素/亚型 | IgG2aκ |
| 控制 | appendix, hepatocellular carcinoma |
| 运输 | wet ice |
| 储存温度 | 2-8℃ |
| 可视化 | cytoplasmic |
