产品说明
一般描述
Histone H4 (UniProt P62805; also known as H4 histone family member A, Histone 1 H4a) is encoded by the HIST1H4A (also known as H4/A, H4FA) gene (Gene ID 8359) in human. Histone H4 is one of the main histone proteins that contribut to the chromatin structure in both prokaryotic and eukaryotic cells. Consecutive methylation of the histone H4 Lys20 (H4K20) is dependent on nucleosome age, and different H4K20 methylation levels are found at distinct nuclear loci. H4K20 monomethylation (H4K20me1) is a repressive mark of transcribed genes, whereas H4K20 trimethylation (H4K20me3) is found in repetitive elements in the human genome. More than 80% of H4K20 is dimethylated in human cells. At least three different methyltransferases (KMTs) have been identified that can catalyze H4K20 methylation, while only lysine demethylases (KDMs) for H4K20me1, but not for H4K20me2 or H4K20me3, have been discovered. The monomethyltransferase gene Setd8 knockout in mouse and in Drosophila is lethal due to defects in cell cycle progression.
特异性
Broad species cross-reactivity expected due to sequence homology.
应用
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected histone H4 Lys20 monomethylation in HeLa cells.
The unconjugated antibody (Cat. No. 07-748) is shown to be suitable also for Immunocytochemistry, Multiplex assay, and Western blotting applications.
Anti-monomethyl Histone H4 (Lys20) Antibody, Alexa Fluor® 488 conjugate is an antibody against monomethyl Histone H4 (Lys20) for use in Immunocytochemistry.
质量
Evaluated by Immunocytochemistry in A431 cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected histone H4 Lys20 monomethylation in A431 cells.
目标描述
~10 kDa observed.
外形
Protein A purified
其他说明
Concentration: Please refer to lot specific datasheet.
法律信息
ALEXA FLUOR is a registered trademark of Life Technologies
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
产品性质
| 生物来源 | rabbit |
| 质量水平 | 100 |
| 偶联物 | ALEXA FLUOR® 488 |
| 抗体形式 | purified antibody |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| species reactivity | human |
| technique(s) | immunocytochemistry: suitable |
| NCBI登记号 | NM_001034077.4 |
| UniProt登记号 | P62805 |
| Gene Information | human ... HIST2H4B(554313) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
