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Anti-JMJD4 (NT) Antibody

品牌
Sigma-Aldrich
货号
07-1538
包装型号
规格纯度
from rabbit
参考价格
6694.05 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Covalent modification of histones plays critical role in regulating chromatin structure and transcription. A family of novel JmjC domain-containing histone demethylation (JHDM) enzymes have been identified that remove specific methyl marks from histones. Histone demethylation by JHDM proteins requires cofactors Fe(II) and alpha-ketoglutarate. Contributions of histone demethylase activity to tumor development, decreases in cell proliferation, and hormone-dependent transcriptional activation have been observed.
JMJD4 is a transcription factor that is a putative histone demethylase. It is highly expressed in lymphoid (spleen) and hemopoietic (bone marrow) tissues

特异性

This antibody recognizes the N-terminal region of human JMJD4.

免疫原

Epitope: N-terminus
The immunogen was a KLH-conjugated synthetic peptide corresponding to the N-terminal region of human JMJD4.

应用

Research Sub Category
Histone Modifying Proteins

Chromatin Biology
Use Anti-JMJD4 (N-terminus) Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC, ELISA to detect JMJD4 (N-terminus) also known as Jumonji domain-containing protein 4.
Research Category
Epigenetics & Nuclear Function
ELISA:
This antibody was reported by an outside laboratory to be suitable for ELISA.

Immunocytochemistry:
HeLa and NIH/3T3 cells incubated with anti-JMJD4 (N-terminus) stained positively on nuclei.

质量

Western Blot Analysis:
1:250 dilution of this antibody detected JMJD4 on 10 µg of A431 lysate

目标描述

~53 kDa

外形

Protein G Chromatography
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Format: Purified

储存及稳定性

Stable for 6 months at 2-8°C from date of receipt. For long term storage, store at -20ºC. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

分析说明

Control
A431 lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

生物来源rabbit
质量水平100
抗体形式purified antibody
antibody product typeprimary antibodies
克隆polyclonal
species reactivitymouse, human
technique(s)ELISA: suitable
immunocytochemistry: suitable
western blot: suitable
NCBI登记号NP_075383.1
UniProt登记号Q9H9V9
运输wet ice

安全信息

储存分类代码10 - Combustible liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

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