产品说明
一般描述
PP2A is a type 2 protein Ser/Thr phosphatase characterized by high activity against the α subunit of Phosphorylase Kinase and low sensitivity to Inhibitor-2. PP2A is distinguishable from PP2B/Calcineurin and PP2C by its partial activity in the absence of divalent cations, and its high sensitivity to Okadaic Acid. It is also thought to be the principal downmodulator of Ser/Thr kinase cascades. PP2A is a heterotrimer containing a structural subunit (A), a regulatory subunit (B), and a catalytic subunit (C). There are multiple isotypes (encoded by distinct genes) of the B subunit, grouped into three families: B/PR55, B′/PR61, and B′′/PR72 . PTPA is a member of the B′ family that is required for activation of PP2A by an undefined mechanism.
特异性
Recognizes protein phosphatase 2A, catalytic subunit.
Peptide is highly conserved in human, rat, bovine, mouse and chicken.
免疫原
Epitope: C-terminus
Synthetic peptide corresponding to a.a. 298-309 of the human sequence.
应用
This Anti-PP2A Antibody, Catalytic subunit is validated for use in WB, IH(P) for the detection of PP2A.
Immunohistochemisty (paraffin): Antibody was diluted to 1:100, using IHC-Select
Research Category
Signaling
Research Sub Category
Kinases & Phosphatases
质量
Routinely evaluated by Western Blot on HEK293 lysates.
Western Blot Analysis:
1:500-1:1,000 dilution is traditonally used to detected PP2A, Catalytic subunit in 10 μg of HEK293 lysates.
目标描述
60 kDa
联系
Replaces: AB1621
外形
Purified rabbit polyclonal IgG in buffer containing PBS with no preservatives.
Affinity purified
储存及稳定性
Stable for 1 year at -80°C from date of receipt. Avoid repeated freeze/thaw.
分析说明
Control
HEK293 cell lysate
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | affinity purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | polyclonal |
纯化方式 | affinity chromatography |
species reactivity | bovine, mouse, chicken, human, rat |
manufacturer/tradename | Upstate® |
technique(s) | immunohistochemistry: suitable (paraffin) western blot: suitable |
同位素/亚型 | IgG |
NCBI登记号 | NM_006245 |
UniProt登记号 | Q14738 |
运输 | dry ice |
Gene Information | human ... PPP2R5D(5528) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |