产品说明
一般描述
Histone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the ′beads on a string′ structure.
特异性
Broad species cross-reactivity is expected.
Recognizes Histone H2A, Mr 14 kDa.
免疫原
KLH-conjugated, synthetic peptide corresponding to amino acids 88-97 (IRNDEELNKL-C) of human Histone H2A.
应用
Anti-Histone H2A (acidic patch) Antibody is a Rabbit Polyclonal Antibody for detection of Histone H2A (acidic patch) also known as histone 2-H2ac, histone IIa & has been validated in ChIP & WB.
Research Sub Category
Histones
Research Category
Epigenetics & Nuclear Function
Immunofluorescence:
Recommend Anti-phospho-Histone H2A.X (Ser139), clone JBW301, catalog #05-636.
质量
Routinely evaluated by western blot on acid extracts from HeLa cell lysate.
Western Blot Analysis:
1:1000-1:5000 dilutions of this lot detected Histone H2A (acidic patch) in acid extracts from HeLa cell lysate.
目标描述
14 kDa
外形
Unpurified
Rabbit antiserum polyclonal IgG in buffer containing 0.05% sodium azide. Frozen solution.
储存及稳定性
Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
分析说明
Control
HeLa cell extract.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | serum |
antibody product type | primary antibodies |
克隆 | polyclonal |
species reactivity | rat, human, chicken, mouse, yeast, Saccharomyces cerevisiae |
manufacturer/tradename | Upstate® |
technique(s) | ChIP: suitable western blot: suitable |
同位素/亚型 | IgG |
NCBI登记号 | NP_003508 |
UniProt登记号 | Q16777 |
运输 | dry ice |
Gene Information | human ... HIST2H2AC(8338) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |