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Anti-FOXO3a/FKHRL1 Antibody

品牌
Sigma-Aldrich
货号
07-702
规格纯度
Upstate®, from rabbit
参考价格
5520.38 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

The forkhead family of transcription factors is a large group of proteins that share a common conserved 100 amino acid DNA binding domain, which is termed a winged-helix or forkhead domain after the founding member of this group, the forkhead gene in Drosophila. In addition to their roles during normal development, recent studies have demonstrated that members of the forkhead family may participate in several kinds of neoplasia. In particular, 3 genes of the human forkhead family, FKHR, FKHRL1, and AFX, which are orthologues of DAF-16 in Caenorhabditis elegans, were identified at the chromosomal breakpoints in human neoplasms. These three genes have been assigned to the FOXO (named for “Forkhead Box, group O”) subfamily of forkhead transcription factors. The forkhead factors, including FKHRL1, regulate their activation through phosphorylation by proto-oncogene Akt (protein kinase B) and/or SGK (serum- and glucocorticoid- induced kinases) in mammalian cells and the phosphorylated form localized at the cytoplasm is recognized to be an inactivated form, whereas nuclear-imported dephosphorylated FKHRL1 is functional as an active transcriptional factor.

特异性

Not tested on other species.
Recognizes FKHRL1/FOXO3A, Mr 90-95 kDa.

免疫原

Epitope: a.a. 330-673
GST fusion protein corresponding to residues 330-673 of human FKHRL1/FOXO3A.

应用

Research Sub Category
Transcription Factors
Use Anti-FOXO3a/FKHRL1 Antibody (Rabbit Polyclonal Antibody) validated in WB to detect FOXO3a/FKHRL1 also known as AF6q21 protein, Forkhead in rhabdomyosarcoma-like 1, forkhead box O3A.
Research Category
Epigenetics & Nuclear Function

质量

Routinely evaluated by Western Blot on 3T3/A31 cell lysates.

Western Blot Analysis:
A 0.5 g/mL dilution of this antibody was used to detect FOXO3a/FKHRL1 in 3T3/A31 cell lysate.

目标描述

Approx. 90-95 kDa

联系

Replaces: 04-1007

外形

Antigen affinity purified rabbit polyclonal, followed by anti-GST depletion, in 0.1M Tris-Glycine (pH7.4), 150mM NaCl with 0.05% sodium azide.
Format: Purified
Affinity purified

储存及稳定性

Stable for 1 year at 2-8ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.

分析说明

Control
3T3/A31 Cell Lysate.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

生物来源rabbit
质量水平100
抗体形式affinity isolated antibody
antibody product typeprimary antibodies
克隆polyclonal
species reactivitymouse, human
manufacturer/tradenameUpstate®
technique(s)western blot: suitable
同位素/亚型IgG
NCBI登记号NM_001455
UniProt登记号O43524
运输wet ice

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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