产品说明
一般描述
Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the ′beads on a string′ structure.
Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).
特异性
Recognizes Histone H4 acetylated on lysine 12.
Broad species cross-reactivity expected
免疫原
peptide containing the sequence (LGAcKGG) corresponding to lysine 12 acetylation of yeast Histone H4.
应用
Use Anti-acetyl-Histone H4 (Lys12) Antibody (Rabbit Polyclonal Antibody) validated in ChIP, DB, WB, ChIP-seq to detect acetyl-Histone H4 (Lys12) also known as H4K12Ac, Histone H4 (acetyl K12).
Research Sub Category
Histones
Research Category
Epigenetics & Nuclear Function
质量
routinely evaluated by immunoblot on acid extracted proteins from HeLa cells
目标描述
10 kDa
外形
Depleted rabbit antiserum in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
Immunodepleted Serum
储存及稳定性
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
分析说明
Control
Acid extracted proteins from HeLa cells treated with sodium butyrate
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
产品性质
| 生物来源 | rabbit |
| 质量水平 | 100 |
| 抗体形式 | serum |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| species reactivity | human, Saccharomyces cerevisiae |
| manufacturer/tradename | Upstate® |
| technique(s) | ChIP: suitable (ChIP-seq) dot blot: suitable western blot: suitable |
| 同位素/亚型 | IgG |
| NCBI登记号 | NM_175054 |
| UniProt登记号 | P62805 Q6DRA9 |
| 运输 | dry ice |
| Gene Information | human ... HIST2H4B(554313) |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 1 |
