产品说明
一般描述
Histone H3 is one of the five main histone proteins that are involved in the structure of chromatin in eukaryotic cells. The chromatin fiber is composed on repetitive units, known as nucleosomes, which are comprised of an octamer of core histones (two each of H2A, H2B, H3, and H4), around which DNA are wrapped. Histone H3 features a main globular domain and a long N-terminal tail. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 is specifically phosphorylated during both mitosis and meiosis in patterns that are specifically coordinated in space and time. These phosphorylations are initiated at different phases of the cell division. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.
特异性
Other homologies: Broad species cross-reactivity expected based on sequence similarity.
Antibody recognizes histone H3 with acetylated Lys9 and/or Lys14.
免疫原
Synthetic peptide corresponding to human histone H3 N-terminal sequence with acetylated Lys9 and Lys14
Epitope: N-terminus
应用
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected acetyl-Histone H3 in NIH/3T3 cells.
Research Sub Category
Nuclear Receptors
Research Category
Epigenetics & Nuclear Function
This Anti-acetyl-Histone H3 Antibody, Alexa Fluor® 647 Conjugate is validated for use in Immunocytochemistry for the detection of acetyl-Histone H3.
质量
Evaluated by Immunocytochemistry in A431 cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected acetyl-Histone H3 in A431 cells.
目标描述
17 kDa observed
外形
Purified rabbit antibody conjugate in buffer containing PBS, 15 mg/mL BSA and 0.1% Sodium Azide.
Protein A purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
其他说明
Concentration: Please refer to lot specific datasheet.
法律信息
ALEXA FLUOR is a registered trademark of Life Technologies
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
产品性质
| 生物来源 | rabbit |
| 质量水平 | 100 |
| 偶联物 | ALEXA FLUOR® 647 |
| 抗体形式 | purified antibody |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| species reactivity | mouse, human |
| species reactivity (predicted by homology) | rat (based on 100% sequence homology) |
| technique(s) | immunocytochemistry: suitable |
| NCBI登记号 | NP_003484.1 |
| UniProt登记号 | Q16695 |
| 运输 | wet ice |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
