产品说明
一般描述
Frizzled 7 is a multi-pass membrane protein belonging to the G-protein coupled receptor Fz/Smo family. It is a receptor for Wnt signaling, predominantly Wnt11, a crucial factor in the regulation of cellular migration and polarization during vertebrate gastrulation. Highest reported expression is in fetal kidney tissue and adult heart tissue. Lower levels have been observed in brain, placenta, adult heart, fetal lung, and squamous cell esophageal carcinomas.
特异性
Recognizes Frizzled-7
免疫原
KLH-conjugated linear peptide corresponding to human Frizzled-7.
Epitope: Unknown
应用
Western Blot Analysis: A previous lot detected Frizzled-7 on 10 µg of HEK293 cell lysate and rat brain microsomal fraction tissue lysate.
Immunohistochemistry Analysis: 1:300 dilution from a previous lot detected Frizzled-7 in human ductal in situ and GI tract tissue.
Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected Frizzled-7 in A431 cells.
Research Category
Signaling
This Anti-Frizzled-7 Antibody is validated for use in WB, IH, IC for the detection of Frizzled-7.
Research Sub Category
Developmental Signaling
质量
Evaluated by Western Blot in mouse brain microsomal fraction tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Frizzled-7 on 10 µg of mouse brain microsomal fraction tissue lysate.
目标描述
The calculated molecular weight is 64 kDa Observed at ~83 kDa, most likely due to multiple glycosolation sites.
外形
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Affinity purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Control
Mouse brain microsomal fraction tissue lysate
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
产品性质
| 生物来源 | rabbit |
| 质量水平 | 100 |
| 抗体形式 | affinity isolated antibody |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 纯化方式 | affinity chromatography |
| species reactivity | human, mouse, rat |
| technique(s) | immunocytochemistry: suitable immunohistochemistry: suitable western blot: suitable |
| NCBI登记号 | NP_003498.1 |
| UniProt登记号 | O75084 |
| 运输 | wet ice |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
