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Anti-Cathepsin D Antibody

品牌
Sigma-Aldrich
货号
06-114
规格纯度
from rabbit, purified by affinity chromatography
参考价格
6190.47 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Cathepsin D is synthesized as a 54kDa precursor which is proteolytically processed to an intermediate 48kDa single chain which matures into more stable 34kDa and 14kDa two chain form. It is an estrogen-regulated lysosomal protease that has been suggested to facilitate cancer cell migration and invasion by digesting the basement membrane, extracellular matrix, and connective tissue. Because of its mitogenic and proteolytic activities, it has been implicated as a prognostic marker in many tumor types, especially breast cancer. Cathepsin D is expressed in epithelial cells as well as in macrophages. Immunoreactivity of cancer cells and tissue infiltrating macrophages must be recorded separately.

特异性

This antibody recognizes Cathepsin D.
84% sequence homology to mouse and rat.

免疫原

Epitope: Heavy Chain
GST-tagged recombinant protein corresponding to human Cathepsin D at the heavy chain.

应用

Research Sub Category
Apoptosis - Additional
Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected Cathepsin D in A431 and HeLa cell lystes.
Anti-Cathepsin D Antibody is an antibody against Cathepsin D for use in WB & IC.
Research Category
Apoptosis & Cancer

Metabolism

质量

Evaluated by Western Blot in A431 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Cathepsin D on 10 µg of A431 cell lysate.

目标描述

Observed molecular weight at ~58 kDa Protein is glycosylated and may run slightly higher than predicted.
Predicted molecular weight of the active form is ~46 kDa and ~ 54 kDa for the precursor form.

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
A431 cell lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

生物来源rabbit
质量水平100
抗体形式affinity isolated antibody
antibody product typeprimary antibodies
克隆polyclonal
纯化方式affinity chromatography
species reactivityhuman
technique(s)immunocytochemistry: suitable
western blot: suitable
NCBI登记号NP_001900
UniProt登记号P07339
运输wet ice

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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