产品说明
特异性
Highly specific for the intermediate filament protein vimentin which is present in all cells of mesenchymal origin. This antibody is positive with cells of mesenchymal derivation, including endothelial cells and certain smooth muscle cells of the vascular tract, fibroblasts, connective tissue, all types of blood cells, including thymocytes, interstitial and testicular Sertoli cells, ovarian follicle cells. It can also be used to detect coexpression of vimentin in combination with other intermediate filament proteins. VIM 3B4 has turned out to be the most avid monoclonal antibody to vimentin. Tumors specifically detected: sarcoma (including myosarcoma), lymphoma, melanoma. The binding region of monoclonal antibody VIM3B4 has been characterized by Bohn et al. (1992). According to these authors, the epitope has been localized on the alpha-helical part of vimentin (rod domain coil 2). Due to an aa substitution at position of aa 353 in murine vimentin (that could explain for the weak cross-reaction of the antibody with murine vimentin) they were able to narrow down the binding region around position 353. These findings were confirmed by truncation mutagenesis experiments using human vimentin (Rogers et al., 1995). Polypeptide reacting: MW 57 kDa intermediate filament protein (vimentin) of mesenchymal cells.
免疫原
Vimentin purified from bovine lens
应用
Research Category
Cell Structure
Western blot
Immunohistochemistry: 1:100; Frozen and paraffin-embedded tissue sections with a minimum of a one hour incubation (longer for paraffin); protease pretreatment is recommended for paraffin-embedded sections.
Immunofluorescence
ELISA
Optimal working dilutions must be determined by the end user.
This Anti-Vimentin Antibody, clone VIM 3B4 is validated for use in ELISA, IF, IH(P), WB for the detection of Vimentin.
Research Sub Category
Cytoskeleton
目标描述
57 kDa
外形
Protein A Purified immunoglobulin presented as lyophilized material. Reconstitute with 1ml distilled water. Final solution is PBS, pH 7.4, containing 0.5% BSA and 0.09% sodium azide.
Format: Purified
Protein A purified
储存及稳定性
Maintain at 2–8°C for up to 12 months from date of receipt.
分析说明
Control
Positive in RD cells, glioma cells, fibroblasts (SV-80), and MDCK cell line
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 100 |
| 生物来源 | mouse |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | VIM 3B4, monoclonal |
| species reactivity | canine, amphibian, bovine, monkey, chicken, human |
| manufacturer/tradename | Chemicon® |
| technique(s) | ELISA: suitable immunofluorescence: suitable immunohistochemistry: suitable (paraffin) western blot: suitable |
| 同位素/亚型 | IgG2a |
| NCBI登记号 | NM_003380.2 |
| UniProt登记号 | P08670 |
| 运输 | wet ice |
| Gene Information | human ... VIM(7431) |
安全信息
| 象形图 |  |
| 警示用语： | Warning |
| 危险声明 | H312 + H332 - H412 |
| 预防措施声明 | P261 - P271 - P273 - P280 - P302 + P352 + P312 - P304 + P340 + P312 |
| 危险分类 | Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 3 |
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 3 |
