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Anti-p53 Antibody, aa 211-220, clone240

品牌
Sigma-Aldrich
货号
CBL404
规格纯度
clone PAb240, Chemicon®, from mouse
参考价格
4609.06 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

p53 was discovered in 1979 as a cellular protein associating with the transforming protein of SV40 tumor virus. Since then, many different biochemical functions have been attributed to the 53 kD phosphoprotein. Experimental evidence has suggested that p53 acts as a negative regulator of cell growth in normal cells (Finlay, 1989). Thus, the inactivation or mutation of p53 may be an essential step in the development of malignancy (Lane and Benchmol, 1990). Wild-type p53 levels in normal cells and tissues were found to be very low. Mutant p53 polypeptide, however, is often found to be present at high concentrations in mammalian tumors and tumor cell lines. For example, in an immuno-histochemistry study 40% of human breast cancer showed elevated levels of mutant p53 in the cell nucleus. Mutations of the p53 protein have some characteristic features:

a) Most of them are missense point mutations giving rise to an altered protein function.

b) Many -but not all- mutant p53 proteins exhibit a common mutant structure, which can be recognized by monoclonal antibodies specific for p53 in the mutant conformation.

特异性

PAb 240 recognizes an epitope of p53 tumor suppressor protein between amino acids 211 and 220 (Gannon, 1990; Legros, 1994) in human, mouse, rat, hamster, monkey, cow and chicken. Assaying native samples (immunoprecipitation, ELISA) the PAb 240 detects only the mutant forms of p53 (Gannon, 1990; Said, 1994). In methods using denatured samples [Western blot analysis (Gannon, 1990) and immunohistochemistry of frozen tissue sections (Said, 1994; Bartek, 1991; Walker, 1991)], the PAb 240 recognizes both mutant and denatured wild-type p53.

免疫原

Epitope: aa 211-220
Murine p53-beta galactosidase fusion protein expressed in E. coli.

应用

Research Sub Category
Transcription Factors
Detect p53 with Anti-p53 Antibody, aa 211-220, clone240 (Mouse Monoclonal Antibody), that has been shown to work in IP, WB & IHC.
Detection of p53 oncogene protein

Detection of mutant p53

Prevalence of detection using CBL 404

-50% colon carcinoma sections positive (30 samples)

-70% lung carcinoma sections positive (50 samples)

-30% carcinoma breast samples positive (50 samples)

Normal and pre-malignant tissues negative

Reacts on methacarn fixed tissue

Optimal working dilutions must be determined by the end user.
Research Category
Epigenetics & Nuclear Function

联系

Replaces: 04-1083

外形

Format: Purified
Purified mouse monoclonal in buffer containing 0.1M Tris-glycine (pH 7.4), 0.15M NaCl, with 0.05% sodium azide. We recommend that each laboratory determine an optimum working titre for use in its particular application.

储存及稳定性

For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

质量水平100
生物来源mouse
抗体形式purified antibody
antibody product typeprimary antibodies
克隆PAb240, monoclonal
species reactivitybovine, monkey, chicken, hamster, human, mouse, rat
manufacturer/tradenameChemicon®
technique(s)immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型IgG1
适用性not suitable for immunohistochemistry (Paraffin)
NCBI登记号NP_000537
UniProt登记号P04637
运输wet ice

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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