产品说明
免疫原
Recombinant fragment corresponding to a region within amino acids 285 and 608 of SLIT2 according to AAI17191
应用
Suggested starting dilutions are as follows: IHC-P: 1:100-1:1000, IP: 1:100-1:500, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
生化/生理作用
Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal chord development may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin. In vitro, silences the attractive effect of NTN1 but not its growth-stimulatory effect and silencing requires the formation of a ROBO1-DCC complex. May be implicated in spinal chord midline post-crossing axon repulsion. In vitro, only commissural axons that crossed the midline responded to SLIT2. In the developing visual system appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. In vitro, collapses and repels retinal ganglion cell growth cones. Seems to play a role in branching and arborization of CNS sensory axons, and in neuronal cell migration. In vitro, Slit homolog 2 protein N-product, but not Slit homolog 2 protein C-product, repels olfactory bulb (OB) but not dorsal root ganglia (DRG) axons, induces OB growth cones collapse and induces branching of DRG axons. Seems to be involved in regulating leukocyte migration.
特点和优势
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外形
1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
产品性质
| 生物来源 | rabbit |
| 质量水平 | 100 |
| 偶联物 | unconjugated |
| 抗体形式 | affinity isolated antibody |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 形式 | buffered aqueous solution |
| species reactivity | human, mouse, rat |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable immunoprecipitation (IP): suitable western blot: 500-3000 |
| NCBI登记号 | AAI17191 |
| UniProt登记号 | O94813 |
| 运输 | wet ice |
| 储存温度 | −20℃ |
| Gene Information | human ... SLIT2(9353) |
安全信息
| 象形图 |  |
| 警示用语： | Warning |
| 危险声明 | H317 - H412 |
| 预防措施声明 | P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313 |
| 危险分类 | Aquatic Chronic 3 - Skin Sens. 1 |
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
