产品说明
一般描述
Monoclonal Anti-KLF5 (mouse IgG1 isotype) is derived from the hybridoma KLF-55 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. KLF5 is a DNA-binding transcriptional regulator. This protein contains three independent peptide modules of the C2H2 zinc finger-type. KLF5 protein is expressed in the prostate, kidney, skeletal muscle, and breast. KLF5 gene is also expressed in embryos and is highly expressed in the later stage of fetal development.
Kruppel like factor 5 (KLF5) is encoded by the gene mapped to human chromosome 13q22.1. The encoded protein belongs to kruppel-like factor (KLF) protein family and is mainly expressed in variety of tissues, including lung, colon, intestine and pancreas.
特异性
Monoclonal Anti-KLF5 recognizes human, monkey, dog, hamster, mouse and rat KLF5.
免疫原
synthetic peptide corresponding to a sequence at the C-terminus of human KLF5, conjugated to KLH. The corresponding sequence is identical to monkey, bovine, chicken, rat and mouse KLF5.
应用
Anti-KLF5 antibody, Mouse monoclonal has been used in western blotting and immunofluorescence.
生化/生理作用
KLF5 protein functions through the restoration of cyclin D1, cyclin B1 and Cdc2 gene expression. This protein is also involved in regulating the inhibitory effect of retinoids on cell proliferation. KLF5 protein regulates the pro-proliferative and transforming activities of oncogenic H-ras. KLF5 protein plays a role as a tumor suppressor in intestinal tumorigenesis in vivo. Deletion and down-regulation of KLF5 leads to prostate and breast cancer.
Kruppel like factor 5 (KLF5) has been involved in the regulation of various cellular functions, such as cell cycle, apoptosis, proliferation and differentiation, in both normal and tumor cells. Additionally, it also plays a vital role in maintenance of cardiovascular remodeling. Overexpression of the gene has been observed in various cancer types, including cervical cancer(CC) and colon cancer. Hence, this can be used as a prognostic marker for these cancers.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
储存及稳定性
For continuous use, store at 2-8℃ for up to one month. For extended storage, freeze at -20oC in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| NACRES | NA.41 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| 克隆 | KLF5-55, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~55 |
| species reactivity | human, rat, hamster, monkey, chicken, mouse, canine |
| 浓度 | ~1.0 mg/mL |
| technique(s) | indirect immunofluorescence: 2.5-5.0 μg/mL using methanol/acetone fixed PC3 cells. western blot: 1.0-2.0 μg/mL using extracts of PC3 cells. |
| 同位素/亚型 | IgG1 |
| UniProt登记号 | Q13887 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... KLF5(688) mouse ... Klf5(12224) rat ... Klf5(84410) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
