产品说明
一般描述
Monoclonal Anti-CYBA (mouse IgM isotype) is derived from the hybridoma CB-64 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a sequence at the internal region of human CYBA, conjugated to keyhole limpet hemocyanin (KLH). The small subunit, p22phox subunit, also known as cytochrome b245, alpha polypeptide/light chain (CYBA) is membrane bound,which is expressed in phagocytic, endothelial and vascular smooth muscle cells. This gene is located on human chromosome16q24
免疫原
Synthetic peptide corresponding to a sequence at the internal region of human CYBA .
应用
Monoclonal Anti-CYBA antibody produced in mouse has been used in:
- immunoblotting
- flow cytometry
- immunofluorescence
生化/生理作用
In prostate cancer cells downregulation of cytochrome b245, alpha polypeptide/light chain (CYBA) was reported to inhibited cell proliferation and colony formation, through AKT and ERK1/2 signaling pathways, indicating CYBA has a role in tumor angiogenesis and tumor growth. Mutations in this gene causes an autosomal recessive forms of chronic granulomatous disease (AR220CGD).
外形
Supplied as a solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | CB-64, monoclonal |
| 形式 | buffered aqueous solution |
| species reactivity | bovine, canine, monkey, human, mouse, rat |
| 浓度 | ~1 mg/mL |
| technique(s) | flow cytometry: 5-10 μg/test using HeLa cells. immunoblotting: 0.5-1 μg/mL using whole extracts of HeLa cells. immunofluorescence: 5-10 μg/mL using HeLa cells. |
| 同位素/亚型 | IgM |
| UniProt登记号 | P13498 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... CYBA(1535) mouse ... Cyba(13057) rat ... Cyba(79129) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
