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Anti-PSAT1 Antibody

品牌
Sigma-Aldrich
货号
ABC950
规格纯度
from rabbit, purified by affinity chromatography
参考价格
5688.25 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Phosphoserine aminotransferase (EC 2.6.1.52; UniProt Q9Y617; also known as Phosphohydroxythreonine aminotransferase, PSAT) is encoded by the PSAT1 (also known as NLS2, PSA, PSATD) gene (Gene ID 29968) in human. PSAT1 is a class-V pyridoxal-phosphate-dependent aminotransferase family member that catalyzes the reversible conversion of 3-phosphohydroxypyruvate and to 1-phosphoserine, as well as the conversion of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to 4-phosphonooxy-L-threonine, using, in both cases, L-glutamate as an amine donor, which is converted to 2-oxoglutarate. PSAT1 is involved in serine biosynthesis from glycolytic intermediates and PSAT1 upregulation is reported in non-small cell lung cancer (NSCLC) and colon cancer cells, while PSAT1 knockdoown is shown to suppress the proliferation of MDA-MB-468 and BT-20 breast cancer cells.

特异性

The immunogen sequence is 100% conserved in murine PSAT1 (UniProt Q99K85) and present in both spliced isoforms of human PSAT1 (Uniprot Q9Y617).

免疫原

Epitope: Region between the two pyridoxal phosphate-binding domains.
KLH-conjugated linear peptide corresponding to a sequence within the region between the two pyridoxal phosphate-binding domains of human PSAT1.

应用

This Anti-PSAT1 Antibody is validated for use in Western Blotting, Immunocytochemistry for the detection of PSAT1.
Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected PSAT1 in A431, HeLa, HepG2, and NIH/3T3 cells.
Research Sub Category
Enzymes & Biochemistry
Research Category
Apoptosis & Cancer

质量

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected PSAT1 in 10 µg of Jurkat cell lysate.

目标描述

~40 kDa observed. 40.42 kDa (human isoform 1; Alpha) and 35.19 kDa (human isoform 2; Beta), 40.47 kDa (mouse) calculated.

外形

Affinity purified.
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702

产品性质

生物来源rabbit
质量水平100
抗体形式affinity isolated antibody
antibody product typeprimary antibodies
克隆polyclonal
纯化方式affinity chromatography
species reactivitymouse, human
species reactivity (predicted by homology)rabbit (based on 100% sequence homology)
technique(s)immunocytochemistry: suitable
western blot: suitable
NCBI登记号NP_478059
UniProt登记号Q9Y617

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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