产品说明
一般描述
Polycomb protein group complexes are responsible for the regulation of hundreds of genes in mammals and insects. These proteins are responsible for the assembly and packaging of chromatin and for the induction of methylation on Histone H3. EZH1 is the catalytic subunit of the PRC2/EED-EZH1 complex. This complex methylates Lys-27 of histone H3, which leads to transcriptional repression of the affected target gene. EZH1 is required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in maintaining the identity of embryonic stem cells.
特异性
Other homologies: Porcine (94% sequence homology).
This antibody potentially recognizes all isoforms of EZH1.
免疫原
KLH-conjugated linear peptide corresponding to human EZH1.
应用
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected EZH1 in human lung and kidney tissues.
Research Category
Epigenetics & Nuclear Function
Detect the EZH1 protein using this Anti-EZH1 validated for use in IHC & WB.
Research Sub Category
Histone Modifying Proteins
质量
Evaluated by Western Blot in mouse embryonic stem cell lysate.
Western Blot Analysis: 0.2 µg/mL of this antibody detected EZH1 in 10 µg of mouse embryonic stem cell lysate.
目标描述
~85 kDa observed. Uniprot states 5 isoforms for EZH1. This antibody potentially recognizes all isoforms. Isoform A is 85,271 Daltons. Isoform B is 86,025 Daltons. Isoform C is 80,699 Daltons. Isoform D is 76,952 Daltons. Isoform E is 68,961 Daltons. (Margueron, 2008).
联系
Replaces: 04-1047
外形
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Control
Mouse embryonic stem cell lysate
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
产品性质
| 生物来源 | rabbit |
| 质量水平 | 100 |
| 抗体形式 | affinity isolated antibody |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 纯化方式 | affinity chromatography |
| species reactivity | human, mouse |
| species reactivity (predicted by homology) | rhesus macaque (based on 100% sequence homology), rat (based on 100% sequence homology), bovine (based on 100% sequence homology) |
| technique(s) | immunohistochemistry: suitable western blot: suitable |
| NCBI登记号 | NP_001982 |
| UniProt登记号 | Q92800 |
| 运输 | wet ice |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
