产品说明
一般描述
DNA polymerase beta (EC 2.7.7.7; UniProt P06746; also known as DNA pol beta, DNA polymerase beta subunit) is encoded by the POLB gene (Gene ID 5423) in human. DNA polymerase beta is involved in DNA recombination and base-excision repair (BER). Upon oxidative DNA damage, Polb translocates to the nucleus, where it associates with AP endonuclease 1 (Apex) to carry out DNA repair. Apex makes a single nick 5′ to an abasic site in double-stranded DNA, Polb then inserts the replacement nucleotide(s). Aberrant POLB overexpression results in an increased rate of spontaneous mutagenesis and a highly mutagenic tolerance phenotype. Thirty percent of all tumors are reported to harbor mutations in the POLB gene.
免疫原
KLH-conjugated linear peptide corresponding to the C-terminus of human DNA polymerase beta.
应用
Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected DNA polymerase beta in normal rat testis, normal human kidney, as well as human lung & prostate cancer tissue sections.
This Anti-DNA polymerase beta Antibody is validated for use in Western Blotting, Immunohistochemistry for the detection of DNA polymerase beta.
质量
Evaluated by Western Blotting in human testis tissue lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected DNA polymerase beta in 10 µg of human and mouse testis tissue, Jurkat, and HeLa lysates.
目标描述
~38 kDa observed. Uncharacterized band(s) may appear in some lysates.
外形
Affinity purified
其他说明
Concentration: Please refer to lot specific datasheet.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | affinity isolated antibody |
antibody product type | primary antibodies |
克隆 | polyclonal |
纯化方式 | affinity chromatography |
species reactivity | rat, human, mouse |
species reactivity (predicted by homology) | Xenopus (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology), bovine (based on 100% sequence homology), canine (based on 100% sequence homology), feline (based on 100% sequence homology) |
technique(s) | immunohistochemistry: suitable western blot: suitable |
NCBI登记号 | NP_002681 |
UniProt登记号 | P06746 |
运输 | wet ice |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |