产品说明
一般描述
Anti-BDNF antibody, Mouse monoclonal, (mouse IgG2b isotype) is derived from the BDNF129-13 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized BALB/C mouse. Brain-derived neurotrophic factor (BDNF), also known as Abrineurin, is a secreted dimeric protein, which belongs to the neurotrophin family of growth factors, along with nerve growth factor (NGF) and neurotrophins 3, 4/5 and 6. BDNF is widely expressed in the central nerve system (CNS).
免疫原
Synthetic peptide corresponding to a region located in the mature human BDNF protein conjugated to KLH
应用
Anti-BDNF antibody, Mouse Monoclonal has been used in:
- immunoblotting
- immunohistochemistry
- immunoprecipitation
- immunocytochemistry
生化/生理作用
Brain-derived neurotrophic factor (BDNF) is involved in hippocampal function and verbal episodic memory in humans. BDNF also plays a vital role in vascular function and participates in angiogenesis through the specific receptor tropomyosin-related kinase B (TrkB). Changes in BDNF expression have been extensively investigated in depression, drug addition, schizophrenia, bipolar and anxiety disorders, Huntington′s disease and Rett syndrome. In addition, BDNF is associated with Alzheimer′s disease (AD) and its reduced levels were detected in post-mortem brain tissues of AD patients. It can exert profound effects on development, morphology, synaptic plasticity and brain functions.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
基本信息
| NACRES | NA.41 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| 克隆 | BDNF129-13, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | ~12 kDa |
| species reactivity | human |
| 浓度 | ~1.0 mg/mL |
| technique(s) | immunoblotting: 2-4 μg/mL using lysate of human HEK-293T cells over-expressing human BDNF protein immunocytochemistry: suitable immunohistochemistry: 20-40 μg/mL using pronase retrieved formalin-fixed, paraffin-embedded human cerebellum sections immunoprecipitation (IP): 10-20 μg/mL using lysate of human HEK-293T cells over-expressing human BDNF protein |
| 同位素/亚型 | IgG2b |
| UniProt登记号 | P23560 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... BDNF(627) |
