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Anti-Nanog antibody, Mouse monoclonal

Monoclonal Anti-Nanog 小鼠抗

品牌
Sigma-Aldrich
货号
N3038
规格纯度
clone NNG-811, purified from hybridoma cell culture
参考价格
1303.85 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Nanog is a homeodomain transcription factor expressed in cells of the inner cell mass of early embryos, in embryonic stem cells (ESC), germ cells and in stem/progenitor cells of some tissues. Together with Oct-4, Sox-2, and Rex-1, it is a molecular marker for pluripotent cells and for undifferentiated stem cells. This protein is crucial for the maintenance of pluripotency of ESCs and is down-regulated when ESCs differentiate.
Nanog controls the expression of many ESC genes together with other stem cell transcription factors like Oct-4 and Sox-2. Nanog targets both repressor and activator complexes to regulatory regions of hundreds of genes in the genome. Expression of nanog can be detected primarily in germ cell tumors and in tumors of other cell types. Nanog is an important marker for Seminomas, testicular carcinomas, teratocarcinomas, and germ cell-like tumors in various tissues. Furthermore, it was shown to transform NIH3T3 cells.
Anti-Nanog antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma NNG-811 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with recombinant human Nanog. The human Nanog gene product is a 305 amino acid, 35 kDa protein with a tryptophan repeat domain and two C-terminal trans-activating domains. It is primarily located in the nucleus.

特异性

Monoclonal Anti-Nanog reacts specifically with human Nanog.

免疫原

recombinant human Nanog.

应用

Monoclonal Anti-Nanog antibody is suitable for use in ELISA, immunoblotting (~ 40 kDa), immunocytochemistry and immunoprecipitation.
Immunoblotting: a working antibody concentration of 2-4 mg/mL is recommended using extracts of NT2 cells.
Anti-Nanog antibody, Mouse monoclonal has been used in:

  • enzyme-linked immunosorbent assay (ELISA)
  • immunoblotting
  • immunocytochemistry
  • flow cytometric analysis
  • immunoprecipitation
  • immunofluorescence

外形

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

NACRESNA.41

产品性质

质量水平200
生物来源mouse
偶联物unconjugated
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆NNG-811, monoclonal
形式buffered aqueous solution
分子量 ~40 kDa
species reactivityhuman
包装antibody small pack of 25 μL
浓度~2 mg/mL
technique(s)immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 4-8 μg/mL
同位素/亚型IgG1
UniProt登记号Q9H9S0
运输dry ice
储存温度−20℃
Gene Informationhuman ... NANOG(79923)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable
个人防护装备Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Sigma-Aldrich

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