产品说明
一般描述
Metastasis associated 1 family member 2 (MTA2) is part of the nucleosome remodeling and histone deacetylation complex. This 668 amino acid protein possesses four different domains.
Monoclonal Anti-MTA2 (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide of human MTA2, conjugated to KLH. Human metastasis-associated protein 2 (MTA2) shares 58% homology to human MTA1 and MTA3 proteins, the C-terminus being more divergent than the N-terminus. MTA2 comprises N-terminal bromo-adjacent homology (BAH) and MTA1 homology 2 (ELM2) domain, SANTand zinc finger (ZnF) domain. It also has the nuclear localization region at C-terminus.
免疫原
synthetic peptide corresponding to amino acids 626-641of human MTA2, conjugated to KLH.
应用
Monoclonal Anti-MTA2 antibody produced in mouse has been used in:
- enzyme linked immunosorbent assay (ELISA)
- immunoprecipitation
- immunocytochemistry
- Western blotting
- antibody microarray
生化/生理作用
Metastasis associated 1 family member 2 (MTA2) has been associated with tumor progression and metastasis.
Human metastasis-associated protein 2 (MTA2) interacts with the histone deacetylase-1 HDAC1 and within the nuclear remodeling and deacetylation complexes ATP-dependent helicase -nucleosome remodelling and histone deacetylation (Mi2/NuRD), suggests that these proteins are involved in transcriptional repression. In the p53 pathway, MTA2/MTA1L1 is better known as protein in the deacetylase complexes p53 target protein in the deacetylase complexes (PID). It inhibits p53-dependent transcriptional activation and its growth arrest and apoptosis functionality. showing that deacetylation and functional interaction by the PID/MTA2/MTA1L1 associated nucleosome remodeling deacetylase (NuRD) complex may represent an important pathway to regulated p53 function.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD03097137 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | MTA2-276, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~73 kDa |
| species reactivity | bovine, rat, mouse, human, canine, monkey, hamster |
| technique(s) | immunocytochemistry: suitable immunoprecipitation (IP): suitable indirect ELISA: suitable microarray: suitable western blot: 1-2 μg/mL using 293T total cell extract |
| 同位素/亚型 | IgG1 |
| UniProt登记号 | O94776 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... MTA2(9219) mouse ... Mta2(23942) rat ... Mta2(361724) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
