Anti-Dopamine D₄ Receptor Rabbit pAb

产品说明

一般描述

Anti-Dopamine D4 Receptor, rabbit polyclonal, recognizes ~48-51 kDa dopamine D4 receptor in rat brain. Does not cross-react with other dopamine receptors. It is validated for use in ELISA, WB & IHC.
Recognizes the ~48-51 kDa dopamine D₄ receptor in rat brain. Does not cross-react with other dopamine receptors.
Rabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes the ~48-51 kDa D₄ dopamine receptor.

免疫原

a synthetic peptide corresponding to amino acids 176-185 of human dopamine D₄ receptor
Human

包装

100 μL in Plastic ampoule

应用

ELISA (see comments)

Immunoblotting (1:500)

Immunohistochemistry (1:10,000)

警告

Toxicity: Standard Handling (A)

外形

Undiluted serum.

重悬

Reconstitute the lyophilized antibody with 100 µl PBS containing 10 mg/ml BSA. For further dilution of the antiserum use a similar buffer formulation. Following reconstitution, refrigerate (4°C). Variables associated with assay conditions will dictate the proper working dilution.

分析说明

Positive Control
Rat brain

其他说明

Mignini, F., et al. 2000. J. Autonom. Pharmacol.20, 325.
This antibody has also been reported to work for ELISA. For blocking immunoblot membranes, use ONLY 4% goat serum or evaporated goat′s milk. For immunohistochemistry, a 1:10,000 dilution of the reconstituted serum demonstrated specific staining of rat brain sections including olfactory bulb glomeruli, cortex layer 4, cortex layers 2 and 3 (dendrite staining), cortex layer 5 (apical dendrite staining), and the dentate gyrus of the hippocampus (diffuse staining in the molecular layer, punctuate staining in the granule cell layer).

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

产品性质

安全信息