产品说明
一般描述
Monoclonal Anti-Actin, α-Smooth Muscle-HRP (mouse IgG2a isotype) is derived from the hybridoma 1A4 produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with a synthetic peptide corresponding to a sequence of α-smooth muscle actin, conjugated to KLH. Actin is a highly conserved protein, expressed as six isoforms and represents differentiation markers of muscle tissues.
免疫原
a synthetic peptide corresponding to a sequence at the NH2 terminal synthetic decapeptide of α-smooth muscle actin, conjugated to KLH.
应用
Monoclonal Anti-Actin, α-Smooth Muscle - Peroxidase antibody has been used in:
- direct-immunoblotting
- direct-immunohistochemistry
- direct- enzyme linked immunosorbent assay (ELISA)
- direct staining of tissues and cells
- characterization of stromal cell heterogeneity in various organs and distinguish smooth muscle cells from fibroblasts in mixed cultures.
生化/生理作用
Actin and Myosin are two major cytoskeletal proteins implicated in cell motility, both constitutively expressed in many cells types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. It has been shown that relative proportions of actin isoforms are diverse in smooth muscles of different organs and change within the same population of smooth muscle cells during development, pathological situations and different culture conditions.
外形
Supplied as lyophilized powder. After reconstitution with 0.1 mL of distilled water to a final antibody concentration of ∼ 2 mg/mL, the solution contains 1% BSA, 2.5% trehalose, 0.05% MIT in 0.01 M sodium phosphate buffered saline
制备说明
Reconstitute in 0.1mL of distilled water.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| NACRES | NA.41 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | peroxidase conjugate |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | 1A4, monoclonal |
| 形式 | lyophilized powder |
| 分子量 | antigen ~42 kDa |
| species reactivity | cat, rat, chicken, dog, frog, sheep, human, lizard, rabbit, guinea pig, snail, hamster, crayfish, viper, bovine, goat, mouse |
| technique(s) | direct ELISA: suitable immunoblotting: 2-4 μg/mL using extracts of mouse heart tissues immunohistochemistry: 5-10 μg/mL using human appendix or tonsil tissue sections |
| 同位素/亚型 | IgG2a |
| 运输 | dry ice |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
